Summary
Two divergent strains of adenovirus type 31 were analyzed by neutralization test and restriction endonuclease (RE) patterns in an effort to find the basis for their genetic variability. One strain, isolated from the throat of a child in Maryland during an upper respiratory illness in 1968, was partially neutralized by Ad 31 antisera (to 16-fold lower than homologous titer) while its own antiserum fully neutralized prototype Ad 31 virus, but shared only 9% of comigrating RE fragments with Ad 31 prototype (vs. 30% with Ad 18 prototype); however, PCR tests specific for the inverted terminal repeat (ITR) sequence of Ads 12 and 18 were negative. The other strain, recovered from a stool sample from an infant with diarrhea in Georgia in 1979, was inhibited by Ad 31 antiserum to within 4-fold homologous titer, but shared only 15% of comigrating fragments with Ad 31 prototype (vs. 91% with Ad 18 prototype); ITR-specific PCR tests with this virus were positive for Ad 12/Ad 18. These data suggest that both strains are from separate evolutionary lines of Ad 31 unrelated to all other isolates studied to date by RE analysis, and that the partial neutralization by prototype Ad 31 antisera might represent small mutations in the hexon gene.
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References
Adrian T, Wadell G, Hierholzer JC, Wigand R (1986) DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol 91: 277–290
Adrian T, Wigand R (1989) Genome type analysis of adenovirus 31, a potential causative agent of infant's enteritis. Arch Virol 105: 81–87
Allard A, Girones R, Juto P, Wadell G (1990) Polymerase chain reaction for detection of adenoviruses in stool samples. J Clin Microbiol 28: 2659–2667
Brown M, Petric M, Middleton PJ (1984) Diagnosis of fastidious adenoviruses 40 and 41 in stool specimens. J Clin Microbiol 20: 334–338
Brown M (1990) Laboratory identification of adenoviruses associated with gastroenteritis in Canada from 1983 to 1986. J Clin Microbiol 28: 1525–1529
D'Ambrosio E, Del Grosso N, Chicca A, Midulla M (1982) Neutralizing antibodies against 33 human adenoviruses in normal children in Rome. J Hyg 89: 155–161
Garon CF, Parr RP, Padmanabhan R, Roninson I, Garrison JW, Rose JA (1982) Structural characterization of the adenovirus 18 inverted terminal repetition. Virology 121: 230–239
Hammond GW, Mauthe G, Joshua J, Hannan CK (1985) Examination of uncommon clinical isolates of human adenoviruses by restriction endonuclease analysis. J Clin Microbiol 21: 611–616
Hierholzer JC, Wigand R, Anderson LJ, Adrian T, Gold JWM (1988) Adenovirus from patients with AIDS: a plethora of serotypes and a description of five new serotypes of subgenus D (types 43–47). J Infect Dis 158: 804–813
Hierholzer JC (1989) Diagnostic procedures for viral, rickettsial and chlamydial infections. In: Schmidt NJ, Emmons RW (eds) Adenoviruses. American Public Health Association, Washington DC, pp 219–264
Hierholzer JC, Stone YO, Broderson JR (1991) Antigenic relationship among the 47 human adenoviruses determined in reference horse antisera. Arch Virol 121: 179–197
Hierholzer JC (1992) Adenovirus in the immunocompromised host. Clin Microbiol Rev 5: 262–274
Hirt B (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26: 365–369
Jacobsson PÅ, Johansson ME, Wadell G (1979) Identification of enteric adenovirus by immunoelectrophoresis (IEOP) technique. J Med Virol 3: 307–312
Johansson ME, Brundin M, Adamson L, Grillner L, Landqvist M, Thörner Å, Zweygberg Wigart B (1989) Characterization of two genome types of adenovirus type 31 isoalted in Stockholm during 1987. J Med Virol 28: 63–68
Johansson ME, Brown M, Hierholzer JC, Thörner Å, Ushijima H, Wadell G (1991) Genome analysis of adenovirus type 31 strains from immunocompromised and immunocompetent patients. J Infect Dis 163: 293–299
Johansson ME, Uhnoo I, Kidd AH, Madeley CR, Wadell G (1980) Direct identification of enteric adenovirus, a candidate new serotype, associated with infantile gastroenteritis. J Clin Microbiol 12: 95–100
Krajden M, Brown M, Petrasek A, Middleton PJ (1990) Clinical features of adenovirus enteritis: a review of 127 cases. Pediatr Infect Dis J 9: 636–641
Li QG, Wadell G (1988) The degree of genetic variability among adenovirus type 4 strains isolated from man and chimpanzee. Arch Virol 101: 65–77
Sussenbach JS (1984) The structure of the genome. In: Ginsberg HS (ed) The adenoviruses. Plenum Press, New York London, p 112
Thörner Å, Johansson ME, Hierholzer JC (1992) Restriction endonuclease patterns of adenovirus type 12 and 18. J Virol Methods 39: 101–109
Uhnoo I, Wadell G, Svensson L, Johansson ME (1984) Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol 20: 365–372
Ushijima HJ, Eshita Y, Araki K, Shinozaki T, Togo T, Matsunaga Y (1988) A study of adenovirus gastroenteritis in the Tokyo area. Eur J Pediatr 147: 90–92
Wigand R, Bartha A, Dreizin RS, Esche H, Ginsberg HS, Green M, Hierholzer JC, Kalter SS, McFerran JB, Pettersson U, Russell WC, Wadell G (1982) Adenoviridae: second report. Intervirology 18: 169–176
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Thörner, P.Å., Ahrel-Andersson, M., Hierholzer, J.C. et al. Characterization of two divergent adenovirus 31 strains. Archives of Virology 133, 397–405 (1993). https://doi.org/10.1007/BF01313778
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DOI: https://doi.org/10.1007/BF01313778