Summary
The entry of 2 influenza viruses was compared to the entry of the Ulster strain of Newcastle disease virus (NDV) by measuring the escape rate of preadsorbed virus from neutralization using a microwell assay. The 2-minute entry of Ulster into Madin-Darby bovine kidney (MDBK) cells increased exponentially over the temperature range for fusion of 30–37° C and was prevented by pH 4.6 which corresponded to the pH within endosomes. The 2-hour entry of both influenza viruses into MDBK cells increased exponentially over the temperature range of 0–30° C with Q10 values similar to Q10 for the endocytosis of horse radish peroxidase and their entry times were reduced to less than 10 minutes at pH 4.6. When Madin-Darby canine kidney (MDCK) cells were used the entry of one influenza virus, NIB-8, took 2 minutes whereas the other, A/Jap/Bel, took 2 hours. The reason for this was unknown. NIB-8 contains genes 1, 2, 3, 7, 8 from A/Puerto Rico/8/34 (H1N1) virus and genes 4, 5, 6 from A/Bangkok/1/79 (H3N2) virus. A/Jap/Bel is a triple reassortant which shares genes 3 and 7 with NIB-8 whereas 1, 2, 4, 5 are from A/Japan/305/57 (H2N2) and genes 6, 8 are from A/Bel/42 (H1N1).
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References
Dimmock, N. J.: Initial stages in infection with animal viruses. J. gen. Virology59, 1–22 (1982).
Hay, A. J., Bellamy, A. R., Abraham, G., Skehel, J. J., Brand, C. M., Webster, R. G.: Procedures for characterisation of the genetic material of candidate vaccine strains. In: International Symposium on Influenza Immunization, (II), Geneva 1977 (Developments in Biological Standardization, Vol. 39), 15–24. Basel: S. Karger 1977.
Huang, R. T. C., Rott, R., Klenk, H.-D.: Influenza viruses cause hemolysis and fusion of cells. Virology110, 243–247 (1981).
Lee, P. M., Cherry, R. J., Bächi, T.: Correlation of rotational mobility and flexibility of Sendai virus spike glycoproteins with fusion activity. Virology128, 65–76 (1983).
Lenard, J., Miller, D. K.: Uncoating of enveloped viruses. Cell28, 5–6 (1982).
Marsh, M., Helenius, A.: Adsorptive endocytosis of Semliki forest virus. J. Mol. Biology142, 439–454 (1980).
Matlin, K. S., Reggio, H., Helenius, A., Simons, K.: Infectious entry pathway of influenza virus in a canine kidney cell line. J. Cell Biology91, 601–613 (1981).
Nagai, Y., Hamaguchi, M., Toyoda, T., Yoshida, T.: The uncoating of paramyxoviruses may not require a low pH mediated step. Virology130, 263–268 (1983).
Oxford, J. S., Corcoran, T., Newman, R., Major, D., Schild, G. C.: Biochemical and antigenic analysis using monoclonal antibodies of a series of influenza A (H3N2) and (H1N1) virus reassortant. Vaccine (in press) (1985).
Russell, P. H.: Augmentation of the antibody forming cells response to neuraminidase-treated cells by myxoviruses. Immunology44, 781–787 (1981).
Russell, P. H.: Newcastle disease virus: the effect of monoclonal antibody in the overlay on virus penetration and the immunoselection of variants. J. gen. Virology65, 795–798 (1984).
Russell, P. H., Griffiths, P. C., Cannon, M. J.: A microwell immunoperoxidase test for screening hybridomas and for diagnosing Newcastle disease virus and Sendai virus. J. Immunol. Meth.61, 165–170 (1983).
Russell, P. H., Griffiths, P. C., Goswami, K. K. A., Alexander, D. J., Cannon, M. J., Russell, W. C.: The characterization of monoclonal antibodies to Newcastle disease virus. J. gen. Virology64, 2069–2072 (1983).
Steinman, R. M., Silver, J. M., Cohn, Z. A.: Pinocytosis in fibroblasts. Quantitative studiesin vitro. J. Cell Biology63, 949–969 (1974).
Stephenson, J. R., Hudson, J. B., Dimmock, N. J.: Early events in influenza virus multiplication. II. Penetration of virus into cells at 4° C. Virology86, 264–271 (1978).
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Russell, P.H. Newcastle disease virus and two influenza viruses: Differing effects of acid and temperature on the uptake of infectious virus into bovine and canine kidney cell lines. Archives of Virology 88, 159–166 (1986). https://doi.org/10.1007/BF01310871
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DOI: https://doi.org/10.1007/BF01310871