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Protein crystalloids in the stroma of bean plastids

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Summary

The formation of protein crystalloids in the stroma of bean plastids has been studied. It has been shown that, besides crystallization of the protein already present induced by water loss (which cannot be inhibited by chloramphenicol), the crystalloids also appear when the leaves are fed — but only in light — with very low concentrations of sucrose or glucose for one or two days. In this case their appearance is completely inhibited by simultaneous treatment of the leaves with chloramphenicol but not with cycloheximide. The process of crystalloid formation and disappearance has also been studied. The possibility of their removal from the plastids into the cell vacuoles has been followed and discussed.

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References

  • Berger, C., undJ. Feierabend, 1967: Plastidenentwicklung und Bildung von Photosynthese-Enzymen in etiolierten Roggenkeimlingen. Physiol. Vég.5, 109–122.

    Google Scholar 

  • Björkman, O., 1968: Carboxydismutase activity in shade-adapted and sun-adapted species of higher plants. Physiol. Plant.21, 1–10.

    Google Scholar 

  • Bradbeer, J. W., 1971: Plastid development in primary leaves ofPhaseolus vulgaris. J. exp. Bot.22, 382–390.

    Google Scholar 

  • Butler, W. L., J. De Greef, T. F. Roth, andH. Oelze-Karow, 1972: The influence of carbonylcyanide-m-chlorophenylhydrazone and 3-(3,4-dichlorophenyl)-1,1-dimethylurea on the fusion of primary thylakoids and formation of crystalline fibrils in bean leaves partially greened in far red light. Plant Physiol.49, 102–104.

    Google Scholar 

  • Coulomb, C., etR. Buvat, 1968: Processus de dégénérescence cytoplasmique partielle dans les cellules de jeunes racines deCucurbita pepo. C. R. Acad. Sci. (Paris)267, 843–844.

    Google Scholar 

  • Dassiou, C., andG. Akoyunoglou, 1969: Effect of light on the RuDP-carboxylase activity in etiolated plants of different ages. In: Progress in Photosynthesis Research (Metzner, H., ed.), Vol. III, pp. 1631–1635. Tübingen: H. Laupp jr.

    Google Scholar 

  • Dolzmann, P., undH. Ullrich, 1966: Einige Beobachtungen über Beziehungen zwischen Chloroplasten und Mitochondrien im Palisadenparenchym vonPhaseolus vulgaris. 2. Pflanzenphysiol.55, 165–180.

    Google Scholar 

  • Ellis, R. J., 1969: Chloroplast ribosomes: Stereospecificity of inhibition by chloramphenicol. Science163, 477–478.

    Google Scholar 

  • Filner, B., andA. O. Klein, 1968: Changes in enzymatic activities in etiolated bean seedling leaves after a brief illumination. Plant Physiol.43, 1587–1596.

    Google Scholar 

  • Fineran, B. A., 1971: Ultrastructure of vacuolar inclusions in root tips. Protoplasma72, 1–18.

    Google Scholar 

  • Gunning, B. E. S., 1965: The fine structure of chloroplast stroma following aldehyde osmium-tetroxide fixation. J. Cell Biol.24, 79–93.

    Google Scholar 

  • —,M. W. Steer, andM. P. Cochrane, 1968: Occurrence, molecular structure, and induced formation of the “stromacentre” in plastids. J. Cell Sci.3, 445–456.

    Google Scholar 

  • Ireland, H. M., andJ. W. Bradbeer, 1971: Plastid development in primary leaves ofPhaseolus vulgaris. The effect ofd-threo andl-threo chloramphenicol on the lightinduced formation of enzymes of the photosynthetic carbon pathway. Planta (Berl.)96, 254–261.

    Google Scholar 

  • Kannangara, C. G., 1969: The formation of ribulose diphosphate carboxylase protein during chloroplast development in barley. Plant Physiol.44, 1533–1537.

    Google Scholar 

  • Kawashima, N., andS. G. Wildman, 1970: Fraction I protein. Ann. Rev. Plant Physiol.21, 325–358.

    Google Scholar 

  • Kleinkopf, G. E., R. C. Huffaker, andA. Matheson, 1970: Light-inducedde novo synthesis of ribulose 1,5 diphosphate carboxylase in greening leaves of barley. Plant Physiol.46, 416–418.

    Google Scholar 

  • Lemoine, Y., 1966: Sur l'existence de structures paracrystallines ou fibreuses dans le stroma des chloroplasts de plantules de haricot (“Phaseolus vulgaris” L.). C. R. Acad. Sci. (Paris)263, 105–108.

    Google Scholar 

  • Margulies, M. M., 1970:In vitro protein synthesis by plastids ofPhaseolus vulgaris. V. Incorporation of14C-leucine into a protein fraction containing ribulose 1,5 diphosphate carboxylase. Plant Physiol.46, 136–141.

    Google Scholar 

  • —, andC. Brubaker, 1970: Effect of chloramphenicol on amino acid incorporation by chloroplasts and comparison with the effect of chloramphenicol on chloroplast developmentin vivo. Plant Physiol.45, 632–633.

    Google Scholar 

  • Marty, F., 1971: Différenciation des plastes dans les laticifères d'Euphorbiacharacias L. C. R. Acad. Sci. (Paris)272, 223–226.

    Google Scholar 

  • McMahon, D., andL. Bogorad, 1968: Inhibition of the formation of photosynthetic enzymes by inhibitors of photosynthesis. Plant Physiol.43, 188–192.

    Google Scholar 

  • Perner, E., 1963: Kristallisationserscheinungen im Stroma isolierter Spinat-Chloroplasten guter Erhaltung. Naturwiss.50, 134–135.

    Google Scholar 

  • Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212.

    Google Scholar 

  • Shumway, L. K., T. E. Weier, andC. R. Stocking, 1967: Crystalline structures inVicia faba chloroplasts. Planta (Berl.)76, 182–189.

    Google Scholar 

  • Smillie, R. M., N. S. Scott, D. Graham, A. M. Grieve, andN. F. Tobin, 1968: Formation and function of chloroplast ribosomes inEuglena. Plant Physiol.43, Suppl., S-6.

    Google Scholar 

  • Thomson, W. W., W. M. Dugger, Jr., andR. L. Palmer, 1965: Effects of peroxyacetyl nitrate on ultrastructure of chloroplasts. Bot. Gaz.126, 66–72.

    Google Scholar 

  • — — —, 1966: Effects of ozone on the fine structure of the palisade parenchyma cells of bean leaves. Canad. J. Bot.44, 1677–1682.

    Google Scholar 

  • Vrhovec, B., 1971: Djelovanje nekih bioihibitora na finu gradju plastida (The effect of some bioinhibitors on the fine structure of plastids). M. Sc.-Thesis, Faculty of Sciences, University of Zagreb.

  • Wrischer, M., 1967: Kristalloide im Plastidenstroma. I. Elektronenmikroskopisch-cytochemische Untersuchungen. Planta (Berl.)75, 309–318.

    Google Scholar 

  • —, 1970: Protein crystalloids in plastid stroma. VIIième Congr. Int. Micr. Elect. Grenoble 1970, Vol. III, 191–192.

    Google Scholar 

  • —, andB. Vrhovec, 1972: The effect of cycloheximide on the fine structure of bean chloroplasts. Acta Bot. Croat.31, 55–60.

    Google Scholar 

  • Zandonella, P., 1970: Infrastructure du tissu nectarigène floral deBeta vulgaris L.: le vacuome et la dégradation du cytoplasme dans les vacuoles. C. R. Acad. Sci. (Paris)271, 70–73.

    Google Scholar 

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Wrischer, M. Protein crystalloids in the stroma of bean plastids. Protoplasma 77, 141–150 (1973). https://doi.org/10.1007/BF01287298

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  • DOI: https://doi.org/10.1007/BF01287298

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