Skip to main content
SpringerLink
Log in

Local accumulation of membrane-associated calcium according to cell pattern formation inMicrasterias denticulata, visualized by chlorotetracycline fluorescence

  • Brief Reports
  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Developing cells ofMicrasterias denticulata Bréb. show a characteristic fluorescence of the plasma membrane (or cortical protoplasm) after treatment with chlorotetracycline (CTC), which is known to be an indicator for membrane-bound Ca2+. Depending on the stage of development the fluorescing sites of the young half cell are distributed in a specific pattern which corresponds to cell pattern formation. Therefore growth and thus cytomorphogenesis inMicrasterias seem to be mediated by a patterned accumulation of Ca2+ at the periphery of the differentiating cell. Participation of Ca2+ in a membrane-recognition process responsible for local vesicle incorporation is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Caswell, A. H., 1972: The migration of divalent cations in mitochrondria visualized by a fluorescent chelate probe. L. Membr. Biol.7, 345–364.

    Google Scholar 

  • — 1979: Methods for measuring intracellular calcium. Int. Rev. Cytol.56, 145–181.

    Google Scholar 

  • —,Hutchison, J. D., 1971: Selectivity of chelation to tetracyclines: evidence for special conformation of calcium chelate. Biochem. Biophys. Res. Com.43, 3, 625–630.

    Google Scholar 

  • Chandler, D. E., Williams, J. A., 1978a: Intracellular divalent cation relase in pancreatic acinar cells during stimulus-secretion coupling. I. Use of chlorotetracycline as fluorescent probe. J. Cell Biol.76, 371–385.

    Google Scholar 

  • — — 1978b: Intracellular divalent cation release in pancreatic acinar cells during stimulus-secretion coupling. II. Subcellular localisation of the fluorescent probe chlorotetracycline. J. Cell Biol.76, 386–399.

    Google Scholar 

  • Gratzl, M., 1980: Transport of membranes and vesicle contents during exocytosis. In: Biological chemistry of organelle formation (Bücher, Th., Sebald, W., Weiss, H., eds.). Berlin-Heidelberg-New York: Springer.

    Google Scholar 

  • Jaffe, L. A., Weisenseel, M. H., Jaffe, L. F., 1975: Calcium accumulations within the growing tips of pollen tubes. J. Cell Biol.67, 488–492.

    Google Scholar 

  • Jaffe, L. F., 1979: Control of development by ionic currents. In: Membrane transduction mechanism (Cone, R. A., Dowling, J. E., eds.). New York: Raven Press.

    Google Scholar 

  • Kiermayer, O., 1964: Untersuchungen über die Morphogenese und Zellwandbildung beiMicrasterias denticulata Bréb. Protoplasma59, 382–420.

    Google Scholar 

  • — 1970a: Elektronenmikroskopische Untersuchungen zum Problem der Cytomorphogenese vonMicrasterias denticulata Bréb. I. Allgemeiner Überblick. Protoplasma69, 97–132.

    Google Scholar 

  • — 1970b: Causal aspects of cytomorphogenesis inMicrasterias. Ann. N. Y. Acad. Sci.175, 686–701.

    Google Scholar 

  • — 1973: Feinstrukturelle Grundlagen der Cytomorphogenese. Ber. dtsch. Bot. Ges.86, 287–291.

    Google Scholar 

  • - 1980: Control of morphogenesis inMicrasterias. In: Handbook of Phycological Methods. Developmental and Cytological Methods (Gantt, E., ed.) Cambridge University Press.

  • — 1981: Cytoplasmic basis of morphogenesis inMicrasterias. In: Cytomorphogenesis in Plants (Kiermayer, O., ed.). Wien-New York: Springer.

    Google Scholar 

  • Reiss, H. D., Herth, W., 1978: Visualization of the Ca2+-gradient in growing pollen tubes ofLilium longiflorum with chlorotetracycline fluorescence. Protoplasma97, 373–377.

    Google Scholar 

  • — — 1979: Calcium gradients in tip growing plant cells visualized by chlorotetracycline fluorescence. Planta146, 615–621.

    Google Scholar 

  • Saunders, M. J., Hepler, P. K., 1981: Localisation of membrane associated calcium following cytokinin treatment inFunaria using chlorotetracycline. Planta152, 272–281.

    Google Scholar 

  • Sievers, A., Schnepf, E., 1981: Morphogenesis and polarity of tubular cells with tip growth. In: Cytomorphogenesis in Plants (Kiermayer, O., ed.). Wien-New York: Springer.

    Google Scholar 

  • Täljedal, I. B., 1978: Chlorotetracycline as a fluorescent Ca2+ probe in pancreatic islet cells. Methodological aspects and effects of alloxan, sugars, methylxanthines and Mg2+. J. Cell Biol.76, 652–674.

    Google Scholar 

  • Waris, H., 1950: Cytophysiological studies onMicrasterias. I. Nuclear and cell division. Physiol. Plant.3, 1–16.

    Google Scholar 

  • Weisenseel, M. H., Jaffe, L. F., 1976: The major growth current through lily pollen tubes enters as K+ and leaves as H+. Planta133, 1–7.

    Google Scholar 

  • —,Kicherer, R. M., 1981: Ionic currents as control mechanism in cytomorphogenesis. In: Cytomorphogenesis in Plants (Kiermayer, O., ed.). Wien-New York: Springer.

    Google Scholar 

  • —,Nuccitelli, R., Jaffe, L. F., 1975: Large electrical currents traverse growing pollen tubes. J. Cell Biol.66, 556–567.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Botanik, Universität Salzburg, Lasserstrasse 39, A-5020, Salzburg

    Ursula Meindl

Authors
  1. Ursula Meindl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meindl, U. Local accumulation of membrane-associated calcium according to cell pattern formation inMicrasterias denticulata, visualized by chlorotetracycline fluorescence. Protoplasma 110, 143–146 (1982). https://doi.org/10.1007/BF01281541

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01281541

Keywords

Search

Navigation