Abstract
Adsorption and covalent immobilization of Ig G to a grafted tetrabranched PEO/PPO block copolymer have been studied and related to the temperature-dependent properties of the grafted polymer. The investigation was performed by means of in situ ellipsometry, as well as by ESCA and ELISA measurements. The results show that the copolymer grafted to polystyrene (PS) surface contracts substantially upon increasing the temperature. A close interrelation was found between the properties of the grafted layer and the amount of protein (Ig G) that could be either adsorbed or covalently immobilized to the modified PS surface. By utilizing the reversed temperature phase behavior exhibited by these copolymers a relatively high loading of protein was obtained at temperatures close to the cloud point. By lowering the temperature after immobilization, the grafted layer regains its hydrophilicity and protein-rejecting properties. Thus, problems associated with interaction between bound protein and the underlying solid surface are minimized.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Merrill EW, Salzman EW (1983) J Am Soc Artif Intern Organs 6:60
Lee JH, Andrade JD (1988) In: Andrade JD (ed) Polymer Surface Dynamics, Plenum Press, New York, p 119
Nagaoka S, Nakao A (1990) Biomaterials 11:119
Lee JH, Kopecek J, Andrade JD (1989) J Biomed Mater Res 23:351
Tiberg F, Malmsten M, Linse P, Lindman B (1991) Langmuir 7:2723
Cohen Stuarr MA, Cosgrove T, Vincent B (1986) Adv Colloid Interface Sci 24:143
Holmberg K, Bergström K, Stark M-B (1992) In: Harris M (ed) Biomedical Applications of Polyethylene Glycol Chemistry. Plenum, New York. Chap. 19
Srere PA, Uyeda K (1976) In: Mossbach K (ed) Methods in Enzymology, Vol 44. Academic Press, New York, p 11
Quash GA, Thomas V, Ogier G, Alaoui SE, Delcros J-G, Ripoll H, Roch A-M, Legastelois S, Ripoll J-P (1989) In: Quash GA, Rodwell JD (eds) Covalently Modified Antigens and Antibodies in Diagnosis and Therapy, Marcel Dekker, New York, p 155
Lindman B, Carlsson A, Karlström G, Malmsten M (1990) Adv Colloid Interface Sci 32:183
Linse P, Björling M (1991) Macromolecules 24:6700
Cleasson PM, Kjellander R, Stenius P, Christenson HK (1986) J Chem Soc Faraday Trans 82:2735
Malmsten M, Claesson PM, Perzon E, Pezron I (1990) Langmuir 6:357
Horn D (1980) In: Goethals EJ (ed) Polymeric Amines and Ammonium Salts. Pergamon Press, New York, p 333
Bergström K, Holmberg K, Safranj A, Hoffman AS, Edgell MJ, Hovanes BA, Harris JM (1992) J Biomed Mat Res 26:779
Azzam RMA, Bashara NM (1977) Ellipsometry and Polarized Light. North Holland, Amsterdam
Engström S, Bäckström K (1987) Langmuir 3:568
de Feijter JA, Benjamins J, Veer FA (1978) Biopolymers, 17:1759
Kiss E, Golander C-G, Eriksson J (1987) Prog Colloid Polym Sci 74:113
Van Den Boomgaard Th, Tadros ThF, Lyklema J (1987) J Colloid Interface Sci 116:8
Tadros ThF, Vincent B (1990) J Phys Chem 84:1575
Gölander C-G, Kiss E (1988) J Colloid Interface Sci 121:240
Merrill EW, Salzman EW, Dennison KA, Tay S-W, Pekala RW (1986) In: Nosé Y, Kjellstrand C, Ivanovich P (eds) Progress in Artificial Organs — 1985. SAO Press, Cleveland
Napper DH (1983) In: Otterwill RH, Rowell RL (eds) Polymeric Stabilization of Colloidal Dispersions. Academic Press, London
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tiberg, F., Brink, C., Hellsten, M. et al. Immobilization of protein to surface-grafted PEO/PPO block copolymers. Colloid Polym Sci 270, 1188–1193 (1992). https://doi.org/10.1007/BF01095059
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01095059