Summary
Fifth instar larvae of the tobacco hornworm,Manduca sexta, tolerate 1-h exposures to temperatures as high as 42°C. Above 42°C, survival declines rapidly to 18% at 44°C and 0% at 48°C. As in other insects, the heat-shock response ofManduca sexta involves the induction of synthesis of heat-shock proteins very similar in size to theDrosophila heat-shock proteins (84, 73, 71, 27, 25, 23, and 22 kd). In the epidermis, heat-shock protein synthesis peaks at 42°C, correlating with the heat sensitivity of both the tissue itself and the intact larva. Some heat-shock proteins have different isoelectric forms depending on tissue. Also, the heat-shock proteins are synthesized over a wider range of temperatures in the imaginal discs and the fat body as compared to the epidermis. In contrast to dipteran insects,Manduca sexta does not exhibit a strong repression of non-heat-shock protein synthesis under tolerable conditions.
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Abbreviations
- TCA :
-
trichloroacetic acid
- PAGE :
-
polyacrylamide gel electrophoresis
- AZT :
-
arbitrary Zeitgeber time
- kd :
-
kilodaltons
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Fittinghoff, C.M., Riddiford, L.M. Heat sensitivity and protein synthesis during heat-shock in the tobacco hornworm,Manduca sexta . J Comp Physiol B 160, 349–356 (1990). https://doi.org/10.1007/BF01075665
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DOI: https://doi.org/10.1007/BF01075665