Abstract
The effects of hexavalent chromium (Cr+6) supplied as K2Cr2O7 were studied on asynchronous cultures ofEuglena gracilis Z strain in heterotrophy on lactate medium. At sublethal concentrations, Cr+6 induces only a lengthening of the Lag-Phase in relation to concentration. This action is quite different from Cr+3 action characterized by a lengthening of generation time in relation to Cr+3 concentrations. The study of the Lag-Phase induced by Cr+6 indicates that cells affected are blocked at the beginning of the S phase. Moreover, a rapid disappearance of Cr+6 was observed in the lactate medium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bariaud A, Bonaly J, Delcourt A (1978) Divers aspects de l'action toxique du Cadmium sur les cellules d'Euglena gracilis. Actualités de biochimie marine. Colloque GABIM. La Rochelle: 135–147
Behari J, Chandra SV, Tandon SK (1978) Comparative toxicity of trivalent and hexavalent chromium to rabbits. Acta biol med Germ Band 37:463–467
Ben Bassat D, Gedalia S, Nachman G, Hillei J (1972) Growth of Chlamydomonas in a medium containing mercury. Nature 240:43–44
Ben Bassat D, Mayer AM (1975) Volatilization of mercury by algae. Physiol Plant 33:128–132
Bonaly J, Delcourt A, Mestre JC (1978a) The effects of some metallic ions on the growth ofChlamydomonas variabilis andEuglena gracilis. Mitt Internat Verein Limnol 21:103–109
Bonaly J, Bariaud A, Delcourt A, Mestre JC (1978b) Les effets des ions Cd2+ sur les cellulesd'Euglena gracilis L Cytotoxicité et acquisition d'une résistance. C R Acad Sci Paris D 287:463–466
Chariot (1966) Methodes de la chimie Analytique, 5rd edn. Masson, Paris, 1019 pp
Delcourt A, Mestre JC (1978) The effects of phenylmercuric acetate on the growth ofChlamydomonas variabilis. Bull Environ Contain Toxicol 20:145–148
Falchuk KH, Fawcett DW, Vallée BL (1975) Role of zinc in cell division ofEuglena gracilis. J Cell Sci 17:57–78
Fasulo MP, Bassi M, Donini A (1982) Cytotoxic effects of hexavalent chromium inEuglena gracilis. I. First observations. Protoplasma 110:39–47
Fasulo MP, Bassi M, Donini A (1983) Cytotoxic effects of hexavalent chromium inEuglena gracilis. II. Physiological and ultrastructural studies. Protoplasma 114:35–43
Gray SJ, Sterling K (1950). The tagging of red cells and plasma proteins with radioactive chromium. J Clin Invest 29:1604–1613
Majone F (1977) Effects of potassium dichromate on mitosis of cultured mammalian cells. Caryologia 30 n° 4:469–481
Mertz W (1969) Chromium occurrence and function in biological systems. Physiol Rev 49:163–239
Moulinier H, Mazoyer R, Anguis I (1968) Contribution à l'étude de l'action du chrome sur la croissance des végétaux. An agron Tome 19 vol 5:553–567
Nakamuro K, Yoshikawa K, Sayato Y, Kurata H (1978) Comparative studies of chromosomal aberration and mutagenicity of trivalent and hexavalent chromium. Mutation Res 58:175–181
Nollendorfa A, Pakalne D, Upitis V (1972) Little known trace elements in Chlorella culture. Chromium Latv PSR Zinat Akad vestis 7:33–43
Pickering QH (1980) Chronic toxicity of hexavalent chromium to the fathead Minnow (Pimephales promelas). Arch Environ Contam Toxicol 9:405–413
Roderer G (1979) Hemmung der Cytokinese und Bildung von Reisenzellen bei Poterioochromonas malhanensis durch organische Bleiverbindungen und andere Agenzien. Protoplasma 99:39–51
Rollinson CL (1966) Problems of chromium reactions. In: Andrews Radioactive Pharmaceuticals US Atomic Energy Commission Conference 65111. Oak Ridge TN p. 429
Romanenko VI, Velichko IA (1974) The influence of the chromium ions on activities of bacteria and algae. Biol Vnutr Vod 21:12–15
Ruthven IA, Cairns J (1973) Response of fresh-water protozoan artificial communities to metals. J Protozool 20 (1):127–135
Sagan L (1965) An unusual pattern of tritiated thymidine incorporation inEuglena. J Protozool 12 (1):105–109
Susa N, Furukawa Y, Tsubaki S (1977) Comparative cytotoxic effects of hexavalent and trivalent chromium compounds on cultured cells. Jap J Vet Sci. 39:325–336
Tandon SK, Saxena DK, Gaur JS, Satya V, Chandra (1978) Comparative toxicity of trivalent and hexavalent chromium. Environ Res 15:90–99
Turner MA, Rust RH (1971) Effect of chromium on cros and mineral nutrition of soy beans. Soil Sci Soc Am Proc 35:755–768
US Environmental Protection Agency (1976) Ambient water quality criteria for chromium. Washington DC
Vaidehi BK, Delcourt A, Mestre JC (1974) La toxicité de l'hydroxyde de phenylmercure sur les algues d'eau douce Scenedesmus subspicatus W et W etEuglena gracilis Klebs. C R Acad Sci Paris D 279:1801–1804
Van Der Putte I, Lubbers J, Zolar Z (1981) Effects of pH on uptake, tissue distribution and retention in hexavalent chromium in rainbow trout (Salmo Gairdneri). Aquatic Toxicol I:3–18
Vannini GL, Fasulo MP, Bruni A (1977) First observations on the cytological changes induced inEuglena gracilis by coumarin treatment. Z Pflanzenphysiol 84:183–187
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brochiero, E., Bonaly, J. & Mestre, J.C. Toxic action of hexavalent chromium onEuglena gracilis cells strain Z grown under heterotrophic conditions. Arch. Environ. Contam. Toxicol. 13, 603–608 (1984). https://doi.org/10.1007/BF01056339
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01056339