Abstract
Examination of nascent globin peptides accumulatingin vitro during globin synthesis in rabbit reticulocyte lysates was carried out. A view was supported that nonrandom distribution of codons with different usage frequencies in mRNA may determine the messenger's translation kinetics. Regions of reduced translation of α- and β-globin polypeptide chains were localized, and the cotranslational protein-folding model suggested previously was substantiated. An active conjunction of synthesis and folding of proteins was proposed as one of the main destinations of a translation nonuniformity.
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Krasheninnikov, I.A., Komar, A.A. & Adzhubei, I.A. Nonuniform size distribution of nascent globin peptides, evidence for pause localization sites, and a cotranslational protein-folding model. J Protein Chem 10, 445–453 (1991). https://doi.org/10.1007/BF01025472
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DOI: https://doi.org/10.1007/BF01025472