Abstract
Bovine serum albumin has been covalently labeled with Remazol brilliant blue R to provide a substrate for a convenient spectrophotometric assay for protein precipitants. The blue protein is especially useful for measuring protein precipitation by vegetable tannins because its absorption maximum is at a wavelength where plant pigments exhibit minimum absorption. Blue BSA has been used to determine, by competition experiments, the relative affinity of various proteins for tannins. A procedure for purifying condensed tannin from commercially available quebracho extract is described.
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Asquith, T.N., Butler, L.G. Use of dye-labeled protein as spectrophotometric assay for protein precipitants such as tannin. J Chem Ecol 11, 1535–1544 (1985). https://doi.org/10.1007/BF01012199
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DOI: https://doi.org/10.1007/BF01012199