Abstract
The 2,3-diphosphoglycerate (2,3-DPG) concentration, oxygen half saturation pressure at pH 7.4 (P 50), pH in plasma and red cells, and mean corpuscular hemoglobin concentration (MCHC) of venous blood were determined during unrestricted daily activity (series I) throughout 24 hrs as well as during prolonged bed rest until noon (series II). In series I an almost synchronous diurnal behavior ofP 50, 2,3-DPG, and plasma pH as well as red cell pH became significantly apparent with highest values in the afternoon. The [2,3-DPG] yielded most pronounced alterations, which made up to 13.5% of the average day value. During prolonged recumbency the [2,3-DPG] showed a nonsignificant tendency to decline; theP 50 remained unchanged throughout that period. The possible reason for the missing [2,3-DPG] increase is a reduced change of red cell pH in series II. An influence of a posture dependent aldosterone secretion either directly on the 2,3-DPG metabolism or indirectly via mediating the red cell pH and thus ruling the formation of this organic phosphoris compound is discussed.
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Arturson, G., Robert, M.: Oxygen affinity of whole blood in human subjects. Acta anaesth. scand.45 (Suppl.), 22–25 (1971)
Astrup, P., Rörth, M., Thorshauge, C.: Dependency on acid-base status of oxyhemoglobin dissociation and 2,3-diphosphoglycerate level in human erythrocytes. II. In vivo studies. Scand. J. clin. Lab. Invest.26, 47–52 (1970)
Bauer, Ch., Rathschlag-Schaefer, A. M.: The influence of aldosterone and cortisol on oxygen affinity and cation concentrations of the blood. Resp. Physiol.2, 360–370 (1968)
Böning, D.: Wirkungen eines akuten Sauerstoffmangels auf die Blutelektrolytkonzentrationen bei höhenangepaßten und nichthöhenangepaßten Menschen. Pflügers Arch.314, 217–230 (1970)
Böning, D., Schweigart, U., Kunze, M.: Diurnal variations of protein and electrolyte concentrations and acid-base status in plasma and red cells of normal man. Europ. J. appl. Physiol.32, 239–250 (1974)
Duhm, J.: Effects of 2,3-diphosphoglycerate and other organic phosphate compounds on oxygen affinity and intracellular pH of human erythrocytes. Pflügers Arch.326, 341–356 (1971)
Duhm, J., Gerlach, E.: On the mechanisms of the hypoxia-induced increase of 2,3-diphospho-glycerate in erythrocytes. Pflügers Arch.326, 254–269 (1971)
Krimsky, J.: D-glycerate-2,3-diphosphate. In: Methoden der Enzymatischen Analyse, Vol. II, H. V. Bergmeyer, Hrsg., S. 1397–1399. Weinheim (Bergstr.): Verlag Chemie 1970
Lenfant, C., Torrance, J., English, E., Finch, C. A., Reynafarje, C., Ramos, J., Faura, J.: Effect of altitude on oxygen binding by hemoglobin and on organic phosphate levels. J. clin. Invest.47, 2652–2656 (1968)
Losert, W., Senft, C., Senft, G.: Extrarenale Wirkungen des Aldosterons und der Spirolactone. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.248, 450–463 (1964)
Muller, A. F., Manning, E. L., Riondel., A M.: Influence of position and activity on the secretion of aldosterone. Lancet1958 I, 711–713
Pelletier, N., Ludens, J. H., Fanestil, D. D.: The role of aldosterone in active sodium transport. Arch. intern. Med.129, 248–257 (1972)
Schultz, D., Rathschlag-Schaefer, A. M.: Changes of oxygen affinity and cation concentration in the blood during 24 hrs. Pflügers Arch.307, R 11 (1969)
Schweigart, U., Böning, D., Tibes, U., Hemmer, B.: The influence of physical activity on the 2,3-diphosphoglycerate concentration of erythrocytes and the oxygen dissociation curve in man. In: Erythrocytes, thrombocytes, leucocytes. IInd. Symp. Vienna 1972, E. Gerlach, K. Moser, E. Deutsch, W. Wilmans, eds., pp. 168–170. Stuttgart: Thieme 1973
Severinghaus, J. W.: Blood gas calculator. J. appl. Physiol.21, 1108–1116 (1966)
Streeten, D. H. P., Solomon, A. K.: The effect of ACTH and adrenal steroids on K transport in human erythrocytes. J. gen. Physiol.37 643–661 (1954)
Wolfe, L. K., Gordon, R. D., Island, D. P., Liddle, S. W.: An analysis of factors determining the circadian pattern of aldosterone excretion. J. clin. Endocr.26, 1261–1266 (1966)
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Böning, D., Meier, U., Schweigart, U. et al. Diurnal changes of the 2,3-diphosphoglycerate concentration in human red cells and the influence of posture. Europ. J. Appl. Physiol. 34, 11–17 (1975). https://doi.org/10.1007/BF00999911
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DOI: https://doi.org/10.1007/BF00999911