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Thiamine-dependent enzyme changes in temporal cortex of patients with Alzheimer's disease

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Abstract

Activites of thiamine-dependent enzymes [pyruvate dehydrogenase (PDHC), α-ketoglutarate dehydrogenase (αKGDH), and transketolase (TK)] were measured in autopsied samples of temporal cortex from six patients with Alzheimer's disease and from eight age-matched control subjects who were free from neurological or psychiatric diseases. Times from death to freezing of dissected material at −70°C were matched. Significant decreases in PDHC (decreased by 70%;P<0.01), αKGDH (decreased by 70%; p<0.01), and TK (decreased by 52%;P<0.01) were observed in brain tissue from patients with Alzheimer's disease. In contrast, activities of glutamate dehydrogenase were within normal limits. These findings suggest a possible role for alterations of brain thiamine metabolism or utilization in Alzheimer's disease

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References

  • Bowen, D. M., Wite, P., Spillane, J. A., Goodhart, M. J., Curzon, G., Iwangoff, P., Meier-Ruge, W., and Davidson, A. N. (1979). Accelerated aging or selective neuronal loss as an important cause of dementia.Lancet 1: 11–13.

    Google Scholar 

  • Butterworth, R. F. (1986). Cerebral thiamine-dependent enzyme changes in experimental Wernicke's Encephalopathy.Metab. Brain Dis. 1: 165–175.

    Google Scholar 

  • Butterworth, R. F. (1989). Effects of thiamine deficiency on brain metabolism: Implications for the pathogenesis of the Wernicke-Korsakoff syndrome.Alcohol Alcohol. 24: 271–279.

    Google Scholar 

  • Butterworth, R. F., and Giguère, J. F. (1984). Pyruvate dehydrogenase activity in regions of the rat brain during postnatal development.J. Neurochem. 43: 280–282.

    Google Scholar 

  • Butterworth, R. F., Giguère, J. F., and Besnard, A. M. (1985). Activities of thiamine-dependent enzymes in two experimental models of thiamine-deficiency encephalopathy. 1. The pyruvate dehydrogenase complex.Neurochem. Res. 10: 1417–1428.

    Google Scholar 

  • Butterworth, R. F., Giguère, J. F., and Besnard, A. M. (1986). Activities of thiamine-dependent enzymes in two experimental models of thiamine-deficiency encephalopathy. 2. α-Ketoglutarate dehydrogenase.Neurochem. Res. 11: 567–577.

    Google Scholar 

  • Collerton, D. (1986). Cholinergic function and intellectual decline in Alzheimer's disease.Neuroscience 19: 1–28.

    Google Scholar 

  • Filla, A., De Michele, G., Brescia Morra, V., Palma, V., Di Lauro, A., Di Geronimo, and Campanella, G. (1986). Glutamate dehydrogenase in human brain: Regional distribution and properties.J. Neurochem. 46: 422–424.

    Google Scholar 

  • Foster, N. L., Chase, T. N., Redio, P., Patronas, N. J., Brooks, R. A., and Dichuro, G. (1983). Alzheimer's disease: Focal cortical changes shown by positron emission tomography.Neurology 33: 961–965.

    Google Scholar 

  • Friedland, R. P., Budinger, T. F., Koss, E., and Ober, B. A. (1985). Alzheimer's disease: Anteriorposterior and lateral hemispheric alterations in cortical glucose utilization.Neurosci. Lett. 53: 235–240.

    Google Scholar 

  • Gibson, G. E., and Blass, J. P. (1983). Metabolism and neurotransmission. In Lajtha, A. (ed.),Handbook of Neurochemistry, 2nd ed., Plenum Press, New York, Vol. 35, pp. 633–651.

    Google Scholar 

  • Gibson, G. E., Ksiesak-Reding, H., Sheu, K. F. R., Mykytyn, V., and Blass, J. P. (1984). Correlation of enzymatic, metabolic and behavioural defects in thiamine deficiency and its reversal.Neurochem. Res. 9: 803–814.

    Google Scholar 

  • Gibson, G. E., Sheu, R. F., Blass, J. P., Baker, A., Carlson, K. C., Harding, B., and Perrino, P. (1988). Reduced activities of thiamine-dependent enzymes in the brains and peripheral tissues of patients with Alzheimers disease.Arch. Neurol. 45: 836–840.

    Google Scholar 

  • Giguère, J. F., and Butterworth, R. F. (1987). Activities of thiamine-dependent enzymes in two experimental models of thiamine-deficiency encephalopathy. 3. Transketolase.Neurochem. Res. 12: 305–310.

    Google Scholar 

  • Giguère, J. F., Besnard, A. M., Lavoie, J., Pomier Layrargues, G., and Butterworth, R. F. (1989). Activities of glutamate-related enzymes in autopsied brain tissue from cirrhotic patients with hepatic encephalopathy. In Butterworth, R. F., and Pomier, Layrargues, G. (eds.), Hepatic Encephalopathy: Pathophysiology and Treatment, Humana Press, Clifton, N.J., pp. 435–445.

    Google Scholar 

  • Hakim, A., and Pappius, H. M. (1983). Sequence of metabolic, clinical and histological events in experimental thiamine deficiency.Ann. Neurol. 13: 365–375.

    Google Scholar 

  • Ksiesak-Reding, H., Blass, J. P., and Gibson, G. E. (1982). Studies on the pyruvate dehydrogenase complex in brain with the arylamine acetyltransferase-couple assay.J. Neurochem. 38: 1627–1636.

    Google Scholar 

  • Langlais, P. J., and Mair, R. G. (1990). Protective effects of the glutamate antagonist MK-801 on pyrithiamine-induced lesions and amino acid changes in rat brain.J. Neurosci. 10: 1664–1674.

    Google Scholar 

  • Lavoie, J., Giguère, J. F., Pomier Layrargues, G., and Butterworth, R. F. (1987). Activities of neuronal and astrocytic marker enzymes in autopsied brain tissue of cirrhotic patients with hepatic encephalopathy.Metab. Brain Dis. 2: 283–290.

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193: 265–275.

    Google Scholar 

  • Pearce, B. R., Palmer, A. M., Bowen, D. M.,et al (1984). Neurotransmitter function and atrophy in caudate nucleus in Alzheimer's disease.Neurochem. Pathol. 2: 221–232.

    Google Scholar 

  • Perry, E. K., Perry, R. H., Tomlinson, B. E., Blessed, G., and Gibson, P. H. (1980). Coenzyme A-acetylating enzymes in Alzheimer's disease: Possible cholinergic “compartment” of pyruvate dehydrogenase.Neurosci. Lett. 18: 105–110.

    Google Scholar 

  • Sheu, K. F. R., Lai, J. C. K., and Blass, J. P. (1985). Pyruvate dehydrogenase phosphate (PDHb) phosphatase in brain: Activity, properties and subcellular localizations.J. Neurochem. 40: 1366–1372.

    Google Scholar 

  • Victor, M., Adams, R. D., and Collins, G. H. (1989).The Wernicke-Korsakoff Syndrome and Related Neurological Disorders Due to Alcoholism and Malnutrition, 2nd ed., P. A. Davis, Philadelphia.

    Google Scholar 

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Butterworth, R.F., Besnard, A.M. Thiamine-dependent enzyme changes in temporal cortex of patients with Alzheimer's disease. Metab Brain Dis 5, 179–184 (1990). https://doi.org/10.1007/BF00997071

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