Abstract
NADH:nitrate reductase (EC 1.6.6.1) from squash (Cucurbita maxima Duch., cv. Buttercup) can catalyze the reduction of a ferriphytosiderophore from barley (Hordeum vulgare L. cv. Europa). Maximal activity occurs at pH 6, with an apparentK m andV max of 76 μM and 21 nmol·min-1·(mg protein)-1, respectively. The ferriphytosiderophore strongly inhibits nitrate reduction catalyzed by nitrate reductase at the optimal pH for nitrate reduction, i.e. 7.5. On the contrary, nitrate is a poor inhibitor of ferriphytosiderophore reduction catalyzed by nitrate reductase at the optimal pH for this reaction, pH 6.0. Thus, squash has the potential to assimilate the iron from a ferriphytosiderophore synthesized by another plant.
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References
Becker, J.O., Messens, E., Hedges, R.W. (1985) The influence of agrobactin on the uptake of ferric ion by plants. FEMS Microbiol. Ecol31, 171–175
Campbell, W.H., Smarrelli, J., Jr. (1978) Purification and kinetics of higher plant NADH:nitrate reductase. Plant Physiol.61, 611–616
Campbell, W.H., Smarrelli, J., Jr. (1986) Nitrate reductase: biochemistry and regulation. In: Biochemical basis of plant breeding, pp. 1–39, Neyra, C.A., ed CRC Press, Boca Raton, FL., USA
Castignetti, D., Smarrelli, J., Jr. (1984) Siderophore reduction catalyzed by higher plant NADH:nitrate reductase. Biochem. Biophys. Res. Commun.125, 52–58
Castignetti, D., Smarrelli, J., Jr. (1986) Siderophores, the iron nutrition of plants, and nitrate reductase. FEBS Lett.209, 147–151
Cline, G.R., Reid, C.P., Powell, P.E., Szaniszlo, P.J. (1984) Effects of a hydroxamate siderophore on iron absorption by sunflower and sorghum. Plant Physiol.76, 36–39
Dailey, H.A., Lascelles, J. (1977) Reduction of iron in synthesis of protoheme bySpirillum itersoni and other organisms. J. Bacteriol.129, 815–820
Duss, F., Mozafar, A., Oertli, J.J., Jaeggi, W. (1986) Effect of bacteria on the iron uptake of axenically-cultured roots of Fe-efficient and Fe-inefficient tomatoes (Lycopersicon esculentum Mill). J. Plant Nutr.9, 587–598
Emery, T.E. (1982) Iron metabolism in humans and plants. Am. Sci.70, 626–632
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the Folinphenol reagent. J. Biol. Chem.193, 265–270
Miller, G.W., Pushnik, J.C., Brown, J.C., Emery, T.E., Jolley, V.D., Warnick, K.Y. (1985) Uptake and translocation of iron from ferrated rhodotorulic acid in tomato. J. Plant Nutr.8, 249–264
Orlando, J.A., Neilands, J.B. (1982) Ferrichrome compounds as a source of iron for higher plants. In: Chemistry and biology of hydroxamic acids, pp. 123–129, Kehl, H., ed. S. Karger, Basel
Page, E.R. (1966) Sideramines in plants and their possible role in iron metabolism. Biochem. J.100, 34
Powell, P.E., Szaniszlo, P.J., Cline, G.R., Reid, C.P. (1982) Hydroxamate siderophores in the iron nutrition of plants. J. Plant Nutr.5, 653–673
Redinbaugh, M.G., Campbell, W.H. (1983) Reduction of ferric citrate catalyzed by NADH: nitrate reductase. Biochem. Biophys. Res. Commun.114, 1182–1188
Reid, C.P., Crowley, D.E., Kim, H.J., Powell, P.E., Szaniszlo, P.J. (1984) Utilization of iron by oat when supplied as ferrated synthetic chelate or as ferrated hydroxamate siderophore. J. Plant Nutr.7, 437–447
Römheld, V., Marschner, H. (1986a) Evidence for a specific uptake system for iron phytosiderophores in roots of grasses. Plant Physiol.80, 175–180
Römheld, V., Marschner, H. (1986b) Mobilization of iron in the rhizosphere of different plant species. Adv. Plant Nutr.2, 155–204
Smarrelli, J. Jr., Campbell, W.H. (1979) NADH dehydrogenase activity of higher plant nitrate reductase (NADH). Plant Sci. Lett.16, 139–147
Smarrelli, J., Jr., Castignetti, D. (1986) Iron acquisition by plants: the reduction of ferrisiderophores by higher plant NADH:nitrate reductase. Biochim. Biophys. Acta.882, 337–342
Stutz, E. (1964) Aufnahme von Ferrioxamine B durch Tomatenpflanzen. Experientia20, 430–431
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Smarrelli, J., Castignetti, D. Iron assimilation in plants: reduction of a ferriphytosiderophore by NADH:nitrate reductase from squash. Planta 173, 563–566 (1988). https://doi.org/10.1007/BF00958970
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DOI: https://doi.org/10.1007/BF00958970