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The isoforms of C protein and their distribution in mammalian skeletal muscle

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Summary

A monoclonal antibody that is specific for the slow skeletal muscle isoform of C protein of rabbit muscle has been prepared by immunizing mice with a crude preparation of human myosin. It reacted with the X protein fraction of rabbit skeletal muscle and stained all type I cells in this tissue. It also stained a fraction of the type II cells with varying intensities. The type II cells staining with antibody to slow C protein also stained with a polyclonal antibody prepared against rabbit fast muscle C protein. The type II cells not staining with antibody to slow C protein stained strongly with antibody to fast C protein. In the human skeletal muscle antibody to slow C protein stained all cells whereas antibody to fast C stained only type II cells. It is concluded that the distribution of the isoforms of C protein in adult vertebrate skeletal muscle is more complex than is the case with proteins such as components of the troponin complex.

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Authors and Affiliations

  1. Department of Immunology, University of Birmingham, PO Box 363, B15 2TT, Birmingham, UK

    G. K. Dhoot

  2. Department of Biochemistry, University of Birmingham, PO Box 363, B15 2TT, Birmingham, UK

    M. C. Hales, B. M. Grail & S. V. Perry

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  1. G. K. Dhoot
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  2. M. C. Hales
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  3. B. M. Grail
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  4. S. V. Perry
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Dhoot, G.K., Hales, M.C., Grail, B.M. et al. The isoforms of C protein and their distribution in mammalian skeletal muscle. J Muscle Res Cell Motil 6, 487–505 (1985). https://doi.org/10.1007/BF00712585

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  • DOI: https://doi.org/10.1007/BF00712585

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