Summary
The transcriptional activity of the DNA sequences coding for certain ribosomal proteins has been measured inEscherichia coli. Two partial diploid strains were isolated from matings of arecA -, argD-, aroE- recipient and an Hfr with an origin at 69.5 min. One contained an F′ element carrying the genes from 69.5 to 72.7 min including therpsL locus (72.4) and the second was diploid for the genes from 69.5 to 71.5 min but did not include any mapped ribosomal protein loci. The molecular weights of the plasmids were estimated to be 140 and 110×106 daltons, respectively. The extent of in vivo transcription of the chromosomal genes on the plasmid and the ribosomal protein mRNA fraction of the total cellular RNA were calculated from DNA-RNA liquid hybridization experiments using both DNA and RNA excess procedures. The results indicated a high degree of transcriptional activity concentrated in the ribosomal protein sequences with 83% of the F′ chromosomal sequences transcribed into mRNA products representing about 0.12% of the total cellular RNA.
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Communicated by H.G. Wittmann
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Seals, A.A., Champney, W.S. & Kushner, S.R. Transcription of ribosomal protein genes carried on F′ plasmids ofEscherichia coli . Molec. Gen. Genet. 150, 183–191 (1977). https://doi.org/10.1007/BF00695398
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DOI: https://doi.org/10.1007/BF00695398