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In vivo-Untersuchungen über den Glucose-Abbau beiArenicola marina (Annelida, Polychaeta)

In vivo studies on glucose degradation inArenicola marina (Annelida, Polychaeta)

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Summary

The pathway of carbohydrate degradation was investigated inArenicola marina by injecting14C-U-glucose into the coelom of the worms. After 6 and 12 hrs of anaerobiosis much of the radioactivity was found to be incorporated into succinate and propionate. Large amounts of both labelled metabolites also appeared in the incubation water. In addition, alanine accumulated radioactivity at a very high rate under aerobic as well as anaerobic conditions. No labelled lactate was detected.

14C-bicarbonate was effectively incorporated into malate, succinate, aspartate, glutamate, and alanine—however, at very different rates and depending on the presence or absence of oxygen.

14C-aspartate,14C-glutamate, and14C-alanine gave rise to the same labelled intermediates as14C-glucose and14C-bicarbonate. There were characteristic differences between the 3 aminoacids in the rates of metabolic turnover and in the labelling patterns.

The activities of 12 “key enzymes” of the intermediary metabolism were assayed in extracts prepared from body-wall tissue. The high activities of the transaminases GPT and GOT were particularly remarkable.

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Abbreviations

GADPH:

Glyceraldehyde-P dehydrogenase

GDH:

Glycerol-3-P dehydrogenase

G-6-PDH:

Glucose-6-P dehydrogenase

GluDH:

Glutamate dehydrogenase

GOT:

Glutamate-Oxalacetate transaminase

GPT:

Glutamate-Pyruvat transaminase

HK:

Hexokinase

LDH:

Lactate dehydrogenase

ME:

Malic enzyme (decarboxylierende Malat-dehydrogenase)

ODH:

Octopin dehydrogenase

PEPCK:

Phosphoenolpyruvat carboxykinase

PK:

Pyruvate kinase

SDH:

Succinate dehydrogenase

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Authors and Affiliations

  1. Lehrstuhl für Tierphysiologie, Zoologisches Institut der Universität Münster, Hindenburglatz 55, D-4400, Münster, Bundesrepublik Deutschland

    E. Zebe

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  1. E. Zebe
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Herrn Prof. B. Rensch anläßlich seines 75. Geburtstages gewidmet.

Frau B. Blum und Frau R. Stöckmann danke ich für zuverlässige und kompetente Mitarbeit. Die Arbeit wurde durch die deutsche Forschungsgemeinschaft großzügig unterstützt (Ze 40/13). Das Scintillationsspektrometer verdanken wir der Landesanstalt für Forschung des Landes Nordrhein-Westfalen.

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Zebe, E. In vivo-Untersuchungen über den Glucose-Abbau beiArenicola marina (Annelida, Polychaeta). J Comp Physiol B 101, 133–145 (1975). https://doi.org/10.1007/BF00694154

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  • DOI: https://doi.org/10.1007/BF00694154

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