Changes in the respiratory properties of the blood in the carp,Cyprinus carpio, induced by diurnal variation in ambient oxygen tension

Summary

Effects of diurnal variation in ambient oxygen tension on acid-base balance and blood respiratory properties were investigated in carp (Cyprinus carpio). The carp were subjected to two cycles in ambient$P_{O_2 }$ between about 130 mm Hg and about 12 mm Hg at 17°C (cf. Figs. 1 and 2). The first period of hypoxia was characterized by a non-compensated respiratory alkalosis, i.e. whole blood showed an increase in pH from 7.92 to 8.14. During the second hypoxic period, 24 h later, a significantly smaller respiratory alkalosis was present, whole blood pH changed from 7.95 (the value found during the intermediate return to normoxia) to 8.08. The latter increase was associated with a significant increase of 25% in plasma bicarbonate concentration compared with the first period of hypoxia (Fig. 1, Table 1). The erythrocytic concentrations of hemoglobin and ATP were lowered by about 10%, compared with the normoxic values, during the two episodes of hypoxia, and this was due to a swelling of the erythrocytes during hypoxia. The red cell GTP concentration showed an altogether different change during the O₂ cycling: the absolute concentration of red cell GTP changed to a steady level, 50% below that present at the onset of the experiment and the major part of this change took place between the two hypoxic periods (Fig. 2, Table 2).

The results are discussed with reference to the respiratory function of the blood of carps during subjection to cyclic$P_{O_2 }$ changes in nature.