Summary
Eye muscles were examined histochemically for the presence of horseradish peroxidase (HRP) activity after intravenous injections of large doses of this tracer protein. HRP had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. HRP was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. Accumulation of HRP in perikarya of motor neurons can therefore be the result of a physiological process of pinocytosis at the axon terminals. In this way exogenous macromolecules in the blood can by-pass the blood-brain barrier and reach the lower motor neurons in the CNS.
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Brightman, M. W., Klatzo, I., Olsson, Y., Reese, T. S.: The blood-barrier to proteins under normal and pathological conditions. J. neurol. Sci.10, 215–239 (1970)
Bodian, D.: A reconsideration of the pathogenesis of poliomyelitis. Amer. J. Hyg.55, 414–438 (1952)
Broadwell, R. D., Brightman, M. W.: Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral and cerebral blood. J. comp. Neurol.166, 257–284 (1976)
Graham, R. C., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural correlates by a new technique. J. Histochem. Cytochem.14, 291–302 (1966)
Heuser, J. E., Reese, T. S.: Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction. J. Cell Biol.57, 315–344 (1973)
Holtzman, E., Freeman, A. R., Kashner, L. A.: Stimulation-dependent alterations in peroxidase uptake at lobster neuromuscular junctions. Science173, 733–736 (1971)
Kristensson, K.: Transport of fluorescent protein tracer in peripheral nerves. Acta neuropath. (Berl.)16, 293–300 (1970)
Kristensson, K.: Retrograde axonal transport of protein tracers. In: The use of axonal transport for studies of neuronal connectivity (eds. W. M. Cowan and M. Cuénod), pp. 69–82. Amsterdam-Oxford-New York: Elsevier 1975
Kristensson, K., Olsson, Y.: Retrograde transport of protein. Brain Res.29, 363–365 (1971)
Kristensson, K., Olsson, Y.: Accumulation of protein tracers in pericytes of the central nervous system following systemic injection in immature mice. Acta neurol. scand.49, 189–194 (1973)
Kristensson, K., Olsson, Y.: Retrograde transport of horseradish peroxidase in transected axons. 3. Entry into injured axons and subsequent localization in perikaryon. Brain Res.115, 201–213 (1976)
LaVail, J. H., LaVail, M. M.: The retrograde intraaxonal transport of horseradish peroxidase in the chick visual system: a light and electron microscopic study. J. comp. Neurol.157, 303–358 (1974)
Saito, A., Zacks, S. I.: Ultrastructure of Schwann and perineural sheaths at the mouse neuromuscular junction. Anat. Rec.164, 379–390 (1969)
Schacher, S., Holtzman, E., Hood, D. C.: Synaptic activity of frog retinal photoreceptors. A peroxidase uptake study. J. Cell Biol.70, 178–192 (1976)
Stoeckel, K., Thoenen, H.: Retrograde axonal transport of nerve growth factor: specificity and biological importance. Brain Res.85, 337–341 (1975)
Teichberg, S., Holtzman, E., Crain, S. M., Peterson, E.: Circulation and turnover of synaptic vesicle membrane in cultured fetal mammalian spinal cord neurons. J. Cell Biol.67, 215–230 (1975)
Turner, P. T., Harris, A. B.: Ultrastructure of exogenous peroxidase in cerebral cortex. Brain Res.74, 305–326 (1974)
Westergaard, E., Go, G., Klatzo, I., Spatz, M.: Increased permeability of cerebral vessels to horseradish peroxidase induced by ischemia in mongolian gerbils. Acta neuropath. (Berl.)35, 307–325 (1976)
Zacks, S. I., Saito, A.: Uptake of exogenous horseradish peroxidase by coated vesicles in mouse neuromuscular junctions. J. Histochem. Cytochem.17, 161–170 (1969)
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Kristensson, K. Retrograde axonal transport of horseradish peroxidase. Acta Neuropathol 38, 143–147 (1977). https://doi.org/10.1007/BF00688561
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DOI: https://doi.org/10.1007/BF00688561