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Brain aging in normotensive and hypertensive strains of rats

III. A quantitative study of cerebrovasculature

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Summary

Quantitative histological techniques were used to evaluate age-related alterations in the vascularity and thickness of the cerebral cortex in normotensive and hypertensive strains of rats. In both strains, aging was accompanied by a statistically significant increase in mean minimal luminal diameter (MMLD) of capillaries in all cortical laminae. While the overall depth of the cerebral cortex was significantly decreased in aged rats of both strains, the total number of capillaries in a strip of cortex extending from the pia mater to the underlying white matter did not change between 3 months and 23–24 months. The greatest vascularity was seen in the field occupying 41–60% of the depth below the pial surface. Few differences in the laminar variations in capillary density were observed. The number and MMLD of venules were increased in aged WKY, but the aged hypertensive rats exhibited a decrease in number and MMLD of venules. No significant change in either the number or MMLD of arterioles was observed in aged and/or hypertensive rats. The total brain weight of the hypertensive rats was significantly less than that of normotensive rats at 3 and 12 months of age but no difference was observed between the aged groups. Hypertensive rats demonstrated a significantly decreased relative brain weight (brain weight per 100 g body weight) in all age groups.

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References

  • Amano S (1977) Vascular changes in the brain of spontaneously hypertensive rats: hyaline and fibrinoid degeneration. J Pathol 121: 119–128

    Google Scholar 

  • Appel E (1965) The role of the vascular factor in the distribution of senile changes in the cerebral cortex. In: Luthy F, Bischoff A (eds) Proceedings of the 5th International Congress of Neuropathology. Excerpta Medica Foundation, Amsterdam, pp 543–545

    Google Scholar 

  • Arendt A (1972) Altern des Zentralnervensystems. In: Gottfried H (ed) Handbuch der Allgemeinen Pathologie, Bd 6. Springer, New York, S 490–542

    Google Scholar 

  • Bar T (1978) Morphometric evaluation of capillaries in different laminae of rat cerebral cortex by automatic image analysis. Changes during development and aging. In: Cervos-Navarro J, et al. (eds) Advances in neurology, vol 20. Raven Press, New York, pp 1–9

    Google Scholar 

  • Barron SA, Jacobs L, Kinkel WR (1976) Changes in size of normal lateral ventricles during aging determined by computerized tomography. Neurology (Minneap) 26: 1011–1013

    Google Scholar 

  • Bondareff W (1959) Morphology of the aging nervous system. In: Birren JE (ed) Handbook of aging and the individual. University of Chicago Press, Chicago, pp 136–168

    Google Scholar 

  • Cogan DG, Kuwabara T, Friedman E (1968) Retinal vasculature. Microvasc Res 1: 115–132

    Google Scholar 

  • Corsellis JAN (1976) Some observations on the Purkinje cell population and on brain volume in human aging. In: Terry RD, Gershon S (eds) Neurobiology of aging. Raven Press, New York, pp 205–227

    Google Scholar 

  • Dastur DK, Lane MM, Hansen DB, Kety SS, Butler RN, Perlin S, Sokoloff L (1963) Effects of aging on cerebral circulation and metabolism in man. In: Human aging. A biological and behavioral study. USPHS Publication No. 986, pp 59–76. US Government Printing Office, Washington, DC

    Google Scholar 

  • Dekaban AS, Sadowsky D (1978) Changes in brain weights during the span of human life. Relation of brain weights to body heights and body weights. Ann Neurol 4: 345–356

    Google Scholar 

  • Diamond MC, Johnson RE, Gold MW (1977) Changes in neuron number and size and glia number in the young, adult, and aging rat medial occipital cortex. Behav Biol 20: 409–418

    Google Scholar 

  • Fujishima M, Omae T (1980) Brain blood flow and transit time as related to aging. Gerontology 26: 104–107

    Google Scholar 

  • Giamocelli F, Weiner J, Spiro D (1970) The cellular pathology of experimental hypertension. V. Increased permeability of cerebral arterial vessels. Am J Pathol 59: 133–160

    Google Scholar 

  • Glatt HJ, Henkind P (1979) Aging changes in the retinal capillary bed of the rat. Microvasc Res 18: 1–17

    Google Scholar 

  • Hunziker O, Abdel'Al S, Schulz A (1979) The aging of human cerebral cortex. A stereological characterization of changes in the capillary net. J Gerontol 34: 345–350

    Google Scholar 

  • Kety SS (1956) Human cerebral blood flow and oxygen consumption as related to aging. J Chronic Dis 3: 478–486

    Google Scholar 

  • Klein AW, Michel ME (1977) A morphometric study of the neocortex of young adult and old maze-differentiated rats. Mech Ageing Dev 6: 441–452

    Google Scholar 

  • Knox CA, Oliveira A (1980) Age-related changes in brain weight and relative vascularity of the cerebral cortex in normotensive and hypertensive strains of rats. Anat Rec 196: 100A-111A

    Google Scholar 

  • Knox CA, Yates RD, Chen I-li, Klara PM (1980) Effects of aging on the structural and permeability characteristics of cerebrovasculature in normotensive and hypertensive strains of rats. Acta Neuropathol (Berl) 51: 1–13

    Google Scholar 

  • Kretschmann HJ, Schleicher A, Wingert F, Zilles K, Loblich HJ (1979) Human brain growth in the 19th and 20th century. J Neurol Sci 40: 169–188

    Google Scholar 

  • Krieg WJS (1946) Connections of the cerebral cortex. I. Albino rat. A. Topography of the cortical areas. J Comp Neurol 84: 221–275

    Google Scholar 

  • Lavy S, Melamed E, Bentin S, Cooper G, Rinot Y (1978) Bihemispheric decreases of regional cerebral blood flow in dementia. Correlation with age-matched normal controls. Ann Neurol 4: 445–450

    Google Scholar 

  • Meier-Ruge W, Hunziker O, Iwangoff P, Reichlmeier K, Sandoz P (1978) Alterations of morphological and neurochemical parameters of the brain due to normal aging. In: Nandy K (ed) Senile dementia. A biomedical approach. Elsevier North-Holland Biomedical Press, New York, pp 33–44

    Google Scholar 

  • Naritomi H, Meyer JS, Sakai F, Yamaguchi F, Shaw T (1979) Effects of advancing age on regional cerebral blood flow. Studies in normal subjects and subjects with risk factors for atherothrombotic stroke. Arch Neurol 36: 410–416

    Google Scholar 

  • Ordy JM, Schjeide AO (1973) Univariate and multivariate models for evaluating long-term changes in neurobiological development, maturity, and aging. Prog Brain Res 40: 26–51

    Google Scholar 

  • Ordy JM, Brizzee KR, Kaack B, Hansche J (1978) Age differences in short-term memory and cell loss in the cortex of the rat. Gerontology 24: 276–285

    Google Scholar 

  • Parmacek MS, Fox JH, Harrison WH, Garron DC, Swenie D (1979) Effect of aging on brain respiration and carbohydrate metabolism of CBF1 mice. Gerontology 25: 185–191

    Google Scholar 

  • Patel MS (1977) Age-dependent changes in the oxidative metabolism in rat brain. J Gerontol 32: 643–646

    Google Scholar 

  • Peress N, Kane WC, Aronson SM (1973) Central nervous system findings in a tenth decade autopsy population. Prog Brain Res 40: 473–483

    Google Scholar 

  • Scheibel ME, Scheibel AB (1976) Differential changes with aging in old and new cortices. In: Nandy K, Sherwin J (eds) The aging brain and senile dementia. Plenum Press New York, pp 39–58

    Google Scholar 

  • Scheinberg P, Blackburn I, Rich M, Saslaw M (1953) Effects of aging on cerebral circulation and metabolism. Arch Neurol Psychiatry 70: 77–85

    Google Scholar 

  • Shefer VF (1972) The absolute number of nerve cells in the cerebral cortex in aging and in some mental disorders (senile and vascular dementia, Pick's and Alzheimer's diseases). Zh Nevropatol Psikhiatr 72: 1024–1029

    Google Scholar 

  • Shefer VF (1977) Hippocampal pathology as a possible factor in the pathogenesis of senile dementias. Neurosci Behav Physiol 8: 236–239

    Google Scholar 

  • Shenkin HA, Novak P, Goluboff B, Soffe AM, Bortin L (1953) The effects of aging arteriosclerosis and hypertension upon the cerebral circulation. J Clin Invest 32: 459–465

    Google Scholar 

  • Strehler BL (1976) Introduction. Aging, and the human brain. In: Terry RD, Gerhon S (eds) Neurobiology of aging. Raven Press, New York, pp 1–22

    Google Scholar 

  • Sturrock RR (1977) Quantitative and morphological changes in neurons and neuroglia in the indusium griseum of aging mice. J Gerontol 32: 647–658

    Google Scholar 

  • Sylvia AL, Rosenthal M (1979) Effects of age on brain oxidative metabolism in vivo. Brain Res 165: 235–248

    Google Scholar 

  • Tobian LJ (1977) Experimental models for the study of hypertension. In: Davis J, Laragh JH, Selwyn A (eds) Hypertension. Mechanisms, diagnosis, and management. H. P. Publishing Co., New York, pp 15–24

    Google Scholar 

  • Tomlinson BE, Blessed G, Roth M (1968) Observations on the brains of non-demented old people. J Neurol Sci 7: 331–356

    Google Scholar 

  • Vaughan DW, Vincent JM (1979) Ultrastructure of neurons in the auditory cortex of aging rats. A morphometric study. J Neurocytol 8: 215–228

    Google Scholar 

  • Vaughan WJ, Calvin M (1977) Electrophoretic analysis of brain proteins from young adult and aged mice. Gerontology 23: 110–126

    Google Scholar 

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Authors and Affiliations

  1. Departments of Anatomy and Neurosurgery, Tulane University School of Medicine, 70112, New Orleans, LA, USA

    Craig A. Knox & Andrew Oliveira

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  1. Craig A. Knox
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  2. Andrew Oliveira
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This study was supported by the American Heart Association-Louisana, Inc.

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Knox, C.A., Oliveira, A. Brain aging in normotensive and hypertensive strains of rats. Acta Neuropathol 52, 17–25 (1980). https://doi.org/10.1007/BF00687224

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  • DOI: https://doi.org/10.1007/BF00687224

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