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Alterations in phospholipid bilayers caused by sodium dodecyl sulphate/triton X-100 mixed systems

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Abstract

The mechanisms governing the subsolubilizing and solubilizing interaction of sodium dodecyl sulphate (SDS)/Triton X-100 mixtures and phosphatidylcholine liposomes were investigated. Permeability alterations were detected as a change in 5(6)-carboxy-fluorescein (CF) released from the interior of vesicles and bilayer solubilization as a decrease in the static light-scattered by liposome suspensions. Three parameters were described as the effective surfactant/lipid molar ratios (Re) at which the surfactant system a) resulted in 50% of CF release (Re 50%CF); b) saturated the liposomes (Re SAT;c) led to a complete solubilization of these structures (Re SOL). From these parameters the corresponding surfactant partition coefficientsK 50%CF,K SAT andK SOL were determined. The free surfactant concentrationsS W were lower than the mixed surfactant CMCs at subsolubilizing level, whereas they remained similar to these values during saturation and solubilization of bilayers in all cases. Although theRe increased as the mole fraction of the SDS rose (X SDS), theK parameters showed a maximum atX SDS values of about 0.6, 0.4 and 0.2 forK 50%CF,K SAT andK SOL respectively. Thus, the higher the surfactant contribution in surfactant/lipid system, the lower theX SDS at which a maximum bilayer/water partitioning of mixed surfactant systems added took place and, consequently, the lower the influence of the SDS in this maximum bilayer/water partitioning.

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Abbreviations

PC:

Phosphatidylcholine

PIPES:

piperazine-1,4 bis (2-ethanesulphonic acid)

SDS:

sodium dodecyl sulphate

X SDS :

mole fraction of sodium dodecyl sulphate in the mixed system

CF:

5(6)-carboxyfluorescein

Re :

effective surfactant/lipid molar ratio

Re 50%CF :

effective surfactant/lipid molar ratio for 50% CF release

Re SAT :

effective surfactant/lipid molar ratio for bilayer saturation

Re SOL :

effective surfactant/lipid molar ratio for bilayer solubilization

S W :

surfactant concentration in the aqueous medium

S W, 50%CF :

surfactant concentration in the aqueous medium for 50% CF release

S W, SAT :

surfactant concentration in the aqueous medium for bilayer saturation

S W, SOL :

surfactant concentration in the aqueous medium for bilayer solubilization

S B :

surfactant concentration in the bilayers

K :

bilayer/aqueous phase surfactant partition coefficient

K 50%CF :

bilayer/aqueous phase surfactant partition coefficient for 50% CF release

K SAT :

bilayer/aqueous phase surfactant partition coefficient for bilayer saturation

K SOL :

bilayer/aqueous phase surfactant partition coefficient for bilayer solubilization

PL:

phospholipid TLC-FID, thin-layer chromatography/flame ionization detection system

PI:

polydispersity index

CMC:

critical micellar concentration

r 2 :

regression coefficient

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  1. Departamento de Tensioactivos, Centro de Investigación y Desarrollo (C.I.D.) Consejo Superior de Investigaciones Cientificas (C.S.I.C.), C/Jordi Girona, 18-26, 08034, Barcelona, Spain

    A. de la Maza & J. L. Parra

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  1. A. de la Maza
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de la Maza, A., Parra, J.L. Alterations in phospholipid bilayers caused by sodium dodecyl sulphate/triton X-100 mixed systems. Colloid Polym Sci 274, 253–260 (1996). https://doi.org/10.1007/BF00665642

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  • DOI: https://doi.org/10.1007/BF00665642

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