Abstract
The mechanisms governing the subsolubilizing and solubilizing interaction of sodium dodecyl sulphate (SDS)/Triton X-100 mixtures and phosphatidylcholine liposomes were investigated. Permeability alterations were detected as a change in 5(6)-carboxy-fluorescein (CF) released from the interior of vesicles and bilayer solubilization as a decrease in the static light-scattered by liposome suspensions. Three parameters were described as the effective surfactant/lipid molar ratios (Re) at which the surfactant system a) resulted in 50% of CF release (Re 50%CF); b) saturated the liposomes (Re SAT;c) led to a complete solubilization of these structures (Re SOL). From these parameters the corresponding surfactant partition coefficientsK 50%CF,K SAT andK SOL were determined. The free surfactant concentrationsS W were lower than the mixed surfactant CMCs at subsolubilizing level, whereas they remained similar to these values during saturation and solubilization of bilayers in all cases. Although theRe increased as the mole fraction of the SDS rose (X SDS), theK parameters showed a maximum atX SDS values of about 0.6, 0.4 and 0.2 forK 50%CF,K SAT andK SOL respectively. Thus, the higher the surfactant contribution in surfactant/lipid system, the lower theX SDS at which a maximum bilayer/water partitioning of mixed surfactant systems added took place and, consequently, the lower the influence of the SDS in this maximum bilayer/water partitioning.
Similar content being viewed by others
Abbreviations
- PC:
-
Phosphatidylcholine
- PIPES:
-
piperazine-1,4 bis (2-ethanesulphonic acid)
- SDS:
-
sodium dodecyl sulphate
- X SDS :
-
mole fraction of sodium dodecyl sulphate in the mixed system
- CF:
-
5(6)-carboxyfluorescein
- Re :
-
effective surfactant/lipid molar ratio
- Re 50%CF :
-
effective surfactant/lipid molar ratio for 50% CF release
- Re SAT :
-
effective surfactant/lipid molar ratio for bilayer saturation
- Re SOL :
-
effective surfactant/lipid molar ratio for bilayer solubilization
- S W :
-
surfactant concentration in the aqueous medium
- S W, 50%CF :
-
surfactant concentration in the aqueous medium for 50% CF release
- S W, SAT :
-
surfactant concentration in the aqueous medium for bilayer saturation
- S W, SOL :
-
surfactant concentration in the aqueous medium for bilayer solubilization
- S B :
-
surfactant concentration in the bilayers
- K :
-
bilayer/aqueous phase surfactant partition coefficient
- K 50%CF :
-
bilayer/aqueous phase surfactant partition coefficient for 50% CF release
- K SAT :
-
bilayer/aqueous phase surfactant partition coefficient for bilayer saturation
- K SOL :
-
bilayer/aqueous phase surfactant partition coefficient for bilayer solubilization
- PL:
-
phospholipid TLC-FID, thin-layer chromatography/flame ionization detection system
- PI:
-
polydispersity index
- CMC:
-
critical micellar concentration
- r 2 :
-
regression coefficient
References
Moon KC, Maibach HI (1991) In: Menné T, Maibach HI (eds) Exogenous Dermatoses: Environmental Dermatitis. CRC Press, Boca Raton, FL pp 217–226
Wilhelm KP, Surber C, Maibach HI (1991) J Invest Dermatol 96: 963–967
Braun-Falco O, Korting HC, Maibach HI (1992) In: Braun-Falco O, Korting HC, Maibach HI (eds) Liposome Dermatics, (Griesbach Conference), Springer-Verlag, Berlin, p 301
Wilhelm KP, Surber C, Maibach HI (1991) J Invest Dermatol 97:927–932
Wilhelm KP, Surber C, Maibach HI (1989) Arch Dermatol Res 281:293–295
Kragh-Hansen U, le Maire M, Noël JP, Gulik-Krzywicki T, Møller JV, (1993) Biochemistry 32:1648–1656
Zeidel ML, Nielsen S, Smith BL, Ambudkar SV, Maunsbach AB, Agre P (1994) Biochemistry 33:1606–1615
Lévy D, Gulik A, Bluzat A, Rigaud JL (1992) Biochim Biophys Acta 1107:283–298
Cully DF, Pares PS (1991) Mol Pharmacol 40:326–332
Dubin PL, Principi JM, Smith BA, Fallon MA (1989) J Colloid Interface Sci 127:558–565
Dubin PL, Rigsbee DR, Gan LM, Fallon MA (1988) Macromolecules 21:2555–2559
Dubin PL, Vea MFY, Fallon MA, Thé SS Rigsbee DR, Gan LM (1990) Langmuir 6:1422–1427
Marszall L (1988) Langmuir 4:90–93
Hollang PM (1986) In: Scamehorn JF Ed Phenomena in Mixed Surfactant Systems, ACS Symposium Series, Vol 311. Amer Chem Soc, Washington DC
Ikeda S, Tsunoda MA, Maeda H (1979) J Colloid Interface Sci 70:448–456
Blake-Haskins JC, Scal D, Rheim LD, Robbins CR (1986) J Soc Cosmet Chem 37:199–207
Urbaneja MA, Alonso A, González-Mañas JM, Goñi FM, Partearroyo MA, Tribout M, Paredes S (1990) Biochem J 270:305–308
Lasch J, Hoffmann J, Omelyanenko WG, Klibanov AA, Torchilin VP, Binder H, Gawrisch K (1990) Biochim Biophys Acta 1022:171–180
Inuoe T, Yamahata T, Shimozawa R (1992) J Colloid Interface Sci 149:345–358
Downing DT, Abraham W, Wegner BK, Willman KW, Marshall JL (1993) Arch Dermatol Res 285:151–157
Ruiz J, Goñi FM, Alonso A (1988) Biochim Biophys Acta 937:127–134
Edwards K Almgren M (1989) Langmuir 5:473–478
Kamenka N, El-Amrani M, Appell J, Lindheimer M (1991) J Colloid Interface Sci 143:463–471
Lichtenberg D (1985) Biochim Biophys Acta 821:470–478
De la Maza A, Parra JL (1993) Langmuir 9:870–873
De la Maza A, Parra JL (1994) Colloid Polym Sci 272:721–730
De la Maza, Parra JL (1994) Biochem J 303:907–914
Singleton WS, Gray MS, Brown ML, White JL (1965) J Am Oil Chem Soc 42:53–57
Rosen MJ, Hua XY (1982) J Colloid Interface Sci 86:164–172
Weinstein JN, Ralston E, Leserman LD, Klausner RD, Dragsten P, Henkart P, Blumenthal R (1986) In: G Gregoriadis Ed Liposome Technology, CRC Press, Boca Raton, FL, Vol III, Chap 13
Lasic DD (1994) Liposomes: From Physics to Applications, Elsevier/North-Holland, Amsterdam, pp 63–107
Ackman RG, McLeod CA, Banerjee AK (1990) J of Planar Chrom 3:450–490
Almog S, Litman BJ, Wimley W, Cohen J, Wachtel EJ, Barenholz Y, Ben-Shaul A, Lichtenberg D (1990) Biochemistry 29:4582–4592
American Public Health Association (1985) In: Standard Methods for the Examination of Water and Wastewater, methods 512 B and 512 C, Washington DC, pp 581–588
Lunkenheimer, K, Wantke D (1981) Colloid Polym Sci 259:354–366
Schurtenberger P, Mazer N, Känzig W (1985) J Phys Chem 89:1042–1049
Ruiz MB, Prado A, Goñi FM, Alonso A (1994) Biochim Biophys Acta 1193:301–306
Partearroyo MA, Urbaneja MA, Goñi FM (1992) FEBS Lett 302:138–140
Cox MF, Borys NF, Matson TP (1985) J Am Oil Chem Soc 62:1139–1146
Schubert R, Beyer K, Wolburg H, Schmidt KH (1986) Biochemistry 25:5263–5269
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de la Maza, A., Parra, J.L. Alterations in phospholipid bilayers caused by sodium dodecyl sulphate/triton X-100 mixed systems. Colloid Polym Sci 274, 253–260 (1996). https://doi.org/10.1007/BF00665642
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00665642