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An improved method for cell and spore counts during aerobic cultivation of crystal toxin producingBacillus sphaericus

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Biotechnology Techniques

Summary

A simple improved method including sonication treatment is proposed to determine accurate cell and spore counts forBacillus sphaericus, a microorganism which can form cell aggregates. Sonication (10 watt power output) for 40 seconds after dilution of culture broth was effective in dispersing clumps of cells and spores without disruption. This improved method gave approximately 2 times higher cell and spore counts compared with the conventional counting methods (without sonication).

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Authors and Affiliations

  1. Department of Biotechnology, University of New South Wales, 2052, Sydney, N.S.W., Australia

    Ken Sasaki, Michael A Idachaba & Peter L Rogers

  2. Hiroshima-Denki Institute of Technology, 6-20-1, Nakano, Akiku, 739-03, Hiroshima, Japan

    Ken Sasaki

Authors
  1. Ken Sasaki
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  2. Michael A Idachaba
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  3. Peter L Rogers
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Sasaki, K., Idachaba, M.A. & Rogers, P.L. An improved method for cell and spore counts during aerobic cultivation of crystal toxin producingBacillus sphaericus . Biotechnol Tech 9, 321–326 (1995). https://doi.org/10.1007/BF00638863

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  • DOI: https://doi.org/10.1007/BF00638863

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