Summary
The polymerase chain reaction (PCR) is particularly well suited for the detection of rare sequences. Taking advantage of the recent isolation of sequences associated with stigma self-incompatibility inBrassica oleracea, we used PCR amplifications with primers synthesized to the S6 cDNA sequence, to demonstrate the presence of mRNA homologous to stigmaS-locus gene (SLG) in anthers during early microsporogenesis. In addition, otherS-locus-related (SLR) sequences were shown to be transcribed in sexual as well as in vegetative tissues (roots, leaves), suggesting that the SLG family might be involved not only in pollen-stigma recognition, but more generally in various forms of plant cell signalling processes. This information corroborates the recent discovery of a cDNA-deduced protein kinase from maize roots, whose extracellular receptor displays high homology withBrassica S-locus-specific glycoproteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Detchepare S, Heizmann P, Dumas C (1989) Changes in protein patterns and protein synthesis development inBrassica oleracea. J Plant Physiol 135:129–137
Ebert PR, Anderson MA, Bernatzky R, Altschuler M, Clarke AE (1989) Genetic polymorphism of self-incompatibility in flowering plants. Cell 56:255–262
Gaude T, Dumas C (1987) Molecular and cellular events of self-incompatibility. In: Giles KL, Praskash J (eds) Pollen: cytology and development. Int Rev Cytol 107, Academic Press, Orlando/FL, pp 333–366
Gaude T, Nasrallah ME, Dumas C (1988) Use of monoclonal antibodies to determine putative S products of pollen inBrassica oleracea. Heredity 61:317–318
Gibbs RA, Chamberlain JS (1989) The polymerase chain reaction: a meeting report. Gen Dev 3:1095–1098
Heslop-Harrison J (1968) Pollen wall development. Science 161:230–237
Lalonde BA, Nasrallah ME, Dwyer KG, Chen C-H, Barlon B, Nasrallah JB (1989) A highly conservedBrassica gene with homology to theS-locus-specific glycoprotein structural gene. Plant Cell 1:249–258
Maniatis T, Fritsch EF, Sambrook J (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Nasrallah ME, Nasrallah JB (1986) Molecular biology of self-incompatibility in plants. Trends Genet 2:239–244
Nasrallah JB, Kao T-H, Goldberg ML, Nasrallah ME (1985) cDNA encoding anS-locus-specific glycoprotein formBrassica oleracea. Nature 318:263–267
Nasrallah JB, Kao T-H, Chen C-H, Goldberg ML, Nasrallah ME (1987) Amino-acid sequence of glycoproteins encoded by three alleles of theS-locus ofBrassica oleracea. Nature 326:617–619
Nasrallah JB, Yu S-M, Nasrallah ME (1988) Self-incompatibility genes ofBrassica oleracea: expression, isolation, and structure. Proc Natl Acad Sci USA 85:5551–5555
Nettancourt D de (1977) Incompatibility in angiosperms. In: Frankel R, Gall GAE, Linskens HF (eds) Monographs on theoretical and applied genetics, No. 3. Springer, Berlin, pp 1–230
Ockendon DJ (1974) Distribution of self-incompatibility alleles and breeding structure of open-pollinated cultivars of brussels sprouts. Heredity 33:159–171
Scutt LP, Gates PJ, Gatehouse JA, Boulter D, Croy RRD (1990) A cDNA encoding anS-locus-specific glycoprotein fromBrassica oleracea plants containing the S5 self-incompatibility allele. Mol Gen Genet 220:409–413
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:505–517
Trick M, Flavell RB (1989) A homozygous S-genotype ofBrassica oleracea expresses twoS-like genes. Mol Gen Genet 218:112–117
Walker JG, Zhang R (1990) Relationship of a putative receptor protein kinase from maize to theS-locus glycoproteins ofBrassica. Nature 345:743–746
Author information
Authors and Affiliations
Additional information
Communicated by H.F. Linskens
Rights and permissions
About this article
Cite this article
Guilluy, C.M., Trick, M., Heizmann, P. et al. PCR detection of transcripts homologous to the self-incompatibility gene in anthers ofBrassica . Theoret. Appl. Genetics 82, 466–472 (1991). https://doi.org/10.1007/BF00588600
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00588600