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Differential expression of several E2-type ubiquitin carrier protein genes at different developmental stages inArabidopsis thaliana andNicotiana sylvestris

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Abstract

We characterized three genes encoding different E2-type ubiquitin carrier proteins involved in the ubiquitin-mediated proteolytic pathway:UbcAt3 shows homologies to the yeastCDC34 gene andUbcAt4a andUbcAt4b are two different genes homologous to theUbc1/4/5 subfamily in yeast. Their accumulation was analysed and compared with that of the different families encoding polyubiquitins, as well as the monoubiquitin fusion protein, which is considered as a marker for cell division, during various developmental stages including GO/S transition and senescence of higher plant cells. Our results imply that theseUbc genes are under the control of complex mechanisms, and are differentially regulated, but not necessarily co-regulated with ubiquitin genes. Even the closely relatedUbcAt4a andUbcAt4b genes of the same multigene subfamily are controlled by distinct regulatory mechanisms.

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Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12, rue du Général Zimmer, 67084, Strasbourg-Cedex, France

    Pascal Genschik, Andrée Durr & Jacqueline Fleck

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  1. Pascal Genschik
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  2. Andrée Durr
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  3. Jacqueline Fleck
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Communicated by A. Kondorosi

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Genschik, P., Durr, A. & Fleck, J. Differential expression of several E2-type ubiquitin carrier protein genes at different developmental stages inArabidopsis thaliana andNicotiana sylvestris . Molec. Gen. Genet. 244, 548–556 (1994). https://doi.org/10.1007/BF00583906

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  • DOI: https://doi.org/10.1007/BF00583906

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