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Nitrogen redistribution during grain growth in wheat (Triticum aestivum L.)

I. Peptide hydrolase activity and protein breakdown in the flag leaf, glumes and stem

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Abstract

The activity of a range of endo- and exopeptidase enzymes have been measured in the glumes, flag leaf and stem during the period of grain development in wheat. The enzymes show a sequential pattern of appearance with activity peaks occurring at a number of intervals from anthesis until just prior to the cessation of grain growth. Of the enzymes studied only the haemoglobin- and casein-degrading activity and alanylglycine-dipeptidase activity increased during the period of rapid protein loss, while aminopeptidase, carboxypeptidase and leucyltyrosine dipeptidase reached maximum activity prior to this period.

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References

  • Arnon, D.I.: Copper enzymes in isolated chloroplasts, polyphenol oxidase inBeta vulgaris. Plant Physiol.24, 1–15 (1949)

    CAS  Google Scholar 

  • Collier, M.D., Murray, D.R.: Leucyl-β-naphthylamidase activities in developing seeds and seedlings ofPisum sativum L. Aust. J. Plant Physiol.4, 571–582 (1977)

    CAS  Google Scholar 

  • Dalling, M.J., Boland, G., Wilson, J.H.: Relation between acid proteinase activity and redistribution of nitrogen during grain development in wheat. Aust. J. Plant Physiol.3, 721–730 (1976)

    CAS  Google Scholar 

  • Feller, U.: Changes in nitrogen contents and in proteolytic activities in different parts of field grown wheat ears (Triticum aestivum L.) during maturation. Plant Cell Physiol.19, 1489–1495 (1978)

    CAS  Google Scholar 

  • Feller, U.K., Soong, T-S.T., Hageman, R.H.: Leaf proteolytic activities and senescence during grain development of field grown corn (Zea mays L.). Plant Physiol.59, 290–294 (1977)

    CAS  Google Scholar 

  • Frith, G.J.T., Bruce, D.G., Dalling, M.J.: Distribution of acid proteinase activity in wheat seedlings. Plant Cell Physiol.16, 1085–1091 (1975)

    CAS  Google Scholar 

  • Frith, G.J.T., Gordon, K.H.J., Dalling, M.J.: Proteolytic enzymes in green wheat leaves. I. Isolation on DEAE-cellulose of several proteinases with acid pH optima. Plant Cell Physiol.19, 491–500 (1978a)

    CAS  Google Scholar 

  • Frith, G.J.T., Swinden, L.B., Dalling, J.J.: Proteolytic enzymes in green wheat leaves. II. Purification by affinity chromatography, and some properties of proteinases with acid pH optima. Plant Cell Physiol.19, 1029–1041 (1978b)

    CAS  Google Scholar 

  • Lowry, O.H., Rosebrough, M.J., Farr, A.L., Randall, R.J.: Protein measurement with Folin phenol reagent. J. Biol. Chem.193, 265–275 (1951)

    CAS  PubMed  Google Scholar 

  • Mikola, J., Kolehmainen, L.: Localization and activity of various peptidases in germinating barley. Planta104, 167–177 (1972)

    Article  CAS  Google Scholar 

  • Nair, T.V.R., Grover, H.L., Abrol, Y.P.: Nitrogen metabolism of the upper three leaf blades of wheat at different soil nitrogen levels. II. Protease activity and mobilization of reduced nitrogen to the developing grains. Physiol. Plant.42, 293–300 (1978)

    CAS  Google Scholar 

  • Peoples, M.B., Dalling, M.J.: Degradation of ribulose 1,5-bisphosphate carboxylase by proteolytic enzymes from crude extracts of wheat leaves. Planta138, 153–160 (1978)

    Article  CAS  Google Scholar 

  • Ryan, C.A.: Proteolytic enzymes and their inhibitors in plants. Annu. Rev. Plant Physiol.24, 173–196 (1973)

    Article  CAS  Google Scholar 

  • Salmia, M.A., Mikola, J.J.: Activities of two peptidases in resting and germinating seeds of Scots pine,Pinus sylvestris. Physiol. Plant.33, 261–265 (1975)

    CAS  Google Scholar 

  • Sofield, I., Evans, L.T., Wardlaw, I.F.: The effects of temperature and light on grain filling in wheat. In: Mechanisms of Regulation of Plant Growth, pp. 909–915, Bieleski, R.L., Ferguson, A.R., Creswell, M.M., eds. Bull. 12, Roy. Soc. N.Z., Wellington 1974

    Google Scholar 

  • Sopanen, T., Carfantan, N.: Activities of various peptidases in the senescing petals of tulips. Physiol. Plant.36, 247–250 (1976)

    CAS  Google Scholar 

  • Waters, S.P., Dalling, M.J.: Distribution and characteristics of aminoacyl β-naphthylamidase activities in wheat seedlings. Aust. J. Plant Physiol.6, 595–606 (1979)

    CAS  Google Scholar 

  • Wittenbach, V.A.: Breakdown of ribulose bisphosphate carboxylase and change in proteolytic activity during dark-induced senescence of wheat seedlings. Plant Physiol.62, 604–608 (1978)

    CAS  Google Scholar 

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Waters, S.P., Peoples, M.B., Simpson, R.J. et al. Nitrogen redistribution during grain growth in wheat (Triticum aestivum L.). Planta 148, 422–428 (1980). https://doi.org/10.1007/BF02395309

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  • DOI: https://doi.org/10.1007/BF02395309

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