Summary
Lanthanum is known to be a marker of extracellular spaces. Intracellular organelles are always devoid of stain. But dark and damaged cells may be entered by the marker, as studied at the cellular level, using the electron probe X-ray microanalyser. The technique may be used to detect increased permeability at a stage when no fine structural abnormality is discernible.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blaineau-Peyretti, S., Nichaise, G.: Strontium accumulation in atrial muscle cells. J. Microsc. Biol. Cell. 26, 127–132 (1976)
Caulfield, J.B., Schrag, P.E.: Electron microscopic study of renal calcification. Am. J. Pathol. 44, 3, 365–381 (1964)
Doggenweiler, C.F., Frenk, S.: Staining properties on cell membranes. Proc. Natl. Acad. Sci. 53, 425–430 (1965)
Galle, P.: Analyse chimique ponctuelle des inclusions intracellulaires. Thèse Sci. Paris, 1965
Galle, P.: Les artéfacts en microanalyse par sonde électronique. J. Microsc. Biol. Cell. 22, 315–332 (1975)
Heggtveit, H.A., Herman, L., Mishra, R.K.: Cardiac necrosis and calcification in experimental magnesium deficiency. Am. J. Pathol. 45, 757 (1964)
Humbert, W.: Cytochemistry and X-ray microprobe analysis of the midgut of Tomocerus minor Lubbock (Insecta, Collembola) with special references to the physiological significance of the mineral concretions. Cell Tissue Res. 187, 397–416 (1978)
Lehninger, A.L.: Mitochondria and calcium ion transport. Biochem. J. 119, 129–138, (1970)
Lucht, U., Maunsbach, A.B.: Effect of parathyroid hormone on osteoclasts in vivo. An ultrastructural and histochemical study. Z. Zellforsch. 141, 529–544 (1973)
Matthews, J.L., Martin, J.H., Arsenis, C., Eisenstein, R., Kuettner, K.: The role of mitochondria in intracellular calcium regulation. In: Cellular mechanisms for calcium transfer and homeostasis. Nichols, G., Wasserman, R.H. (eds.), p. 239–252. London, New York: Academic Press 1971
Peachey, L.: Electron microscopic observations on the accumulation of divalent cations in intramitochondrial granules. J. Cell Biol. 20, 95–111 (1964)
Revel, J.P., Karnovsky, M.J.: Hexagonal array of subunits in intracellular junctions of the mouse heart and liver. J. Cell Biol. 33, C7-C11 (1967)
Russ, J.C.: X-ray microanalysis in the biological sciences. J. Submicrosc. Cytol. 6, 55–79 (1974)
Somlyo, A.P., Somlyo, A.V., Devine, C.E., Peters, P.D., Hall, T.A.: Electron microscopy and electron probe analysis of mitochondrial cation accumulation in smooth muscle. J. Cell Biol. 61, 723–742 (1974)
Stuve, J., Galle, P.: Role of the mitochondria in the handling of gold by the kidney. J. Cell Biol. 44, 667–702 (1969)
Sutfin, L.V., Holtrop, M.E., Ogilvie, R.E.: Microanalysis of individual mitochondrial granules with diameters less than 1,000 Angstroms. Science 174, 947–949 (1971)
Thomas, R.S., Greenawalt, J.W.: Microincineration, electron microscopy and electron diffraction of calcium phosphate loaded mitochondria. J. Cell Biol. 39, 55–76 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Humbert, W. Intracellular and intramitochondrial binding of lanthanum in dark degenerating midgut cells of a collembolan (Insect). Histochemistry 59, 117–128 (1978). https://doi.org/10.1007/BF00518507
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00518507