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Improved section adhesion for immunocytochemistry using high molecular weight polymers of l-lysine as a slide coating

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Summary

Poly-l-lysine (PPL) has been used to coat glass slides in the preparation of tissue sections for immunocytochemical staining. The adhesive properties of different molecular weight (m.w.) polymers of l-lysine have been tested on pre-fixed cryostat sections which were subjected to a 3 day washing treatment. It has been found that the higher the molecular weight of the polymer, the greater the adhesive force it provides. PLL (m.w. 350,000) at concentrations in the range of 0.05–0.1% was found to be the most effective polymer.

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Author notes

  1. W. M. Huang (visiting scholar)

    Present address: Institute of Acupuncture, Academy of Traditional Chinese Medicine, Peking, China

  2. J. Gu (visiting scholar)

    Present address: Dept. of Pathology, Peking Medical College, Peking, China

Authors and Affiliations

  1. Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Ducane Road, W12 OHS, London, UK

    W. M. Huang (visiting scholar), S. J. Gibson, P. Facer, J. Gu (visiting scholar) & J. M. Polak

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  1. W. M. Huang
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  2. S. J. Gibson
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  3. P. Facer
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  4. J. Gu
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  5. J. M. Polak
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Huang, W.M., Gibson, S.J., Facer, P. et al. Improved section adhesion for immunocytochemistry using high molecular weight polymers of l-lysine as a slide coating. Histochemistry 77, 275–279 (1983). https://doi.org/10.1007/BF00506570

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  • DOI: https://doi.org/10.1007/BF00506570

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