Summary
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1.
The effects on cutaneous vascular permeability of substance P. physalaemin, eledoisinrelated peptide, bradykinin, somatostatin, neurotensin, methionine enkephalin, vasoactive intestinal peptide, cholecystokinin and pentagastrin were investigated in rats using a quantitative Evans blue dye leakage technique to estimate plasma exudation.
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2.
Substance P, physalaemin, eledoisin-related peptide, bradykinin, somatostatin and neurotensin produced plasma exudation, neurotensin being the most potent of the peptides tested. The high potency of neurotensin would suggest that this peptide might play a role in local regulation of the microcirculation in some organs.
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3.
Pretreatment of rats with mepyramine and methysergide significantly reduced responses to substance P (5×10−9 mol) and neurotensin (5×10−9 mol).
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4.
Responses to neurotensin (5×10−11 and 5×10−10 mol) were almost abolished by mepyramine and methysergide and also be pretreatment of skin sites with compound 48/80. The response to substance P (5×10−10 mol) was not reduced by compound 48/80 but was reduced by mepyramine and methysergide. The response to substance P (5×10−9 mol) was reduced by both treatments but was not abolished.
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5.
No significant interactions were found between any of the peptide combinations tested.
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6.
The effect of neurotensin was significantly potentiated by 5-hydroxytryptamine, PGE1 and ATP.
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7.
It was concluded that substance P has both a direct action to increase vascular permeability in rat skin and an indirect action mediated by amine release from mast cells, whereas neurotensin has mainly an indirect amine-releasing action.
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A preliminary report of this work was given at the 12th annual meeting of the Australasian Society of Clinical and Experimental Pharmacologists, December, 1978
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Chahl, L.A. The effect of putative peptide neurotransmitters on cutaneous vascular permeability in the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 309, 159–163 (1979). https://doi.org/10.1007/BF00501224
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DOI: https://doi.org/10.1007/BF00501224