Skip to main content
Log in

Pore size and properties of spherical Ca-alginate biocatalysts

  • Biotechnology
  • Published:
European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

A method for the production of spherical alginate gels from few μm up to several mm diameter is described. Alginate beads with diameters from 0.5 to 3 mm are used to immobilize microorganisms as biocatalysts. Micro size alginate gels are used as column packing material for inverse steric exclusion chromatography to determine their pore sizes from dextran standards of known molecular weight. The results indicate that alginate gels have pores which are large enough to release catalytic acting enzymes out of the matrix.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Brodelius P, Mosbach K (1982) Immobilized plant cells. Adv Appl Microbiol 28:1–25

    Google Scholar 

  • Buchholz K, Gödelmann B (1979) Exclusion chromatography. Dechema Monogr 84:92–94

    Google Scholar 

  • Cheetham PSJ (1979) Physical studies on the mechanical stability of columns of Calcium alginate pellets containing entrapped microbial cells. Enzyme Microb Technol 1:183–188

    Google Scholar 

  • Fukushima S, Hanai S (1982) Pilot operation for continuous alcohol fermentation of molasses in an immobilized bioreactor. In: Chibata I, Fukui S, Wingard LB Jr. (eds) Enzyme engineering, vol 6. Plenum Press, New York, 347–348

    Google Scholar 

  • Hackel U, Klein J, Megnet R, Wagner F (1975) Immobilization of microbial cells in polymer matrices. Eur J Appl Microbiol Biotechnol 1:291–293

    Google Scholar 

  • Hackel U (1976) Polymereinschluß von Mikroorganismen. Dissertation, TU Braunschweig, FRG

    Google Scholar 

  • Kierstan M, Bucke C (1977) The immobilization of microbial cells, subcellular organelles, and enzymes in Calcium alginate gels. Biotechnol Bioeng 11:387–397

    Google Scholar 

  • Klein J, Wagner F (1978) Immobilized whole cells. Dechema Monogr 82:142–164

    Google Scholar 

  • Klein J, Eng H (1979) Immobilized whole cells. Dechema Monogr 82:142–164

    Google Scholar 

  • Klein J, Eng H (1979) The measurement of the abrasion. Dechema Monogr 84:292–299

    Google Scholar 

  • Klein J, Washausen P (1979a) Pressure stability. Dechema Monogr 84:277–283

    Google Scholar 

  • Klein J, Washausen P (1979b) Diffusion. Dechema Monogr 84:300–302

    Google Scholar 

  • Klein J, Manecke G (1982) New developments in the preparation and characterization of polymerbound biocatalysts. Enzyme Eng 6:181–189

    Google Scholar 

  • Klein J, Vorlop KD (1983) Immobilized cells — catalyst preparation and reaction performance. In: Blanch HW, Papoutsakis ET, Stephanopoulos G (eds) Foundations of biochemical engineering, vol 207/12. ACS Sympos Series, pp 377–392

  • McDowell RH (1977) Properities of alginates. Alginate Industries Limited

  • Messing RA (1974) Simultaneously immobilized glucose oxidase and catalase in controlled — pore titania. Biotechnol Bioeng 16:897–908

    Google Scholar 

  • Messing RA (1975) Immobilization by inorganic bridge formation. Immobilized enzymes for industrial reactors. Academic Press, New York San Francisco London, pp 91–98

    Google Scholar 

  • Polymer Handbook (1974) 2. ed. Wiley & Sons, New York

  • Scherer P, Kluge M, Klein J, Sahm H (1981) Immobilization of the methanogenic bacterium Methanosarcina barkeri. Biotechnol Bioeng 23:1057–1065

    Google Scholar 

  • Schlegel HG (1981) Allgemeine Mikrobiologie. Thieme, Stuttgart, S 320

    Google Scholar 

  • Snyder LR, Kirkland JJ (1974) Introduction to modern liquid chromatography. Wiley & Sons, New York

    Google Scholar 

  • Stock J (1981) Immobilisierung von Aspergillus niger in Alginat-Gelen für die Gluconsäureproduktion und Permeabilität der Matrix. Diplom-Thesis, TU Braunschweig, FRG

    Google Scholar 

  • Vollmert B (1973) Polymer chemistry. Springer, New York

    Google Scholar 

  • Vorlop KD (1978) Immobilisierung von Enzymen und Mikroorganismen durch ionische Vernetzung von Polyelektrolyten. Diplom-Thesis, TU Braunschweig, FRG

    Google Scholar 

  • Vorlop KD, Lein J, Wagner F (1980) Immobilization of microbial cells by polymer precipitation. Abstracts, 6th Intern Ferment Sympos, London (Canada), p 122

  • Vorlop KD, Klein J (1983) New developments in the field of cell immobilization — Formation of biocatalysts by ionotropic gelation. 3rd Rotenburg symposium — Enzyme technology. Springer, New York Wien (in press)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klein, J., Stock, J. & Vorlop, K.D. Pore size and properties of spherical Ca-alginate biocatalysts. European J. Appl. Microbiol. Biotechnol. 18, 86–91 (1983). https://doi.org/10.1007/BF00500829

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00500829

Keywords

Navigation