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Lack of algesic effect of substance P on paravascular pain receptors

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Summary

  1. 1.

    Substance P (synthetic or extracted from intestine or central nervous system) is devoid of an algesic effect on paravascular pain receptors.

  2. 2.

    The algesic effect of a SP-containing acetone HCl-extract from spinal cord is explained by its high content of potassium ions.

  3. 3.

    SP-containing preparations which include an ammonium sulphate precipitation in the extraction procedure are algesic due to content of this salt.

  4. 4.

    SP-containing extract from intestine were found to be contaminated with a bradykinin-like peptide of high algesic potency.

  5. 5.

    These findings are discussed with regard to the restricted value of earlier results about central actions of SP-containing tissue extracts and with regard to the role of SP as a possible neurotransmitter.

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References

  • Amin, A. H., Crawford, T. B. B., Gaddum, J. H.: The distribution of substance P and 5-hydroxytryptamine in the central nervous system of the dog. J. Physiol. (Lond.) 126, 596–618 (1954)

    Google Scholar 

  • Armstrong, D., Dry, R. M. L., Keele, C. A., Markham, J. W.: Observations on chemical excitants of cutaneous pain in man. J. Physiol. (Lond.) 120, 326–351 (1953)

    Google Scholar 

  • Baldauf, J., Gebhardt, K.: Ein Substanz P Standardpräparat. Naunyn-Schmiedeberg's Arch. Pharmak. 265, 278–286 (1969)

    Google Scholar 

  • Bisset, G. W., Lewis, G. P.: A spectrum of pharmacological activity in some biologically active peptides. Br. J. Pharmacol. 19, 168–182 (1962)

    Google Scholar 

  • Braun, C., Guzman, F., Horton, E. W., Lim, R. K. S., Potter, G. D.: Visceral receptors, pain, bradykinin and analgesic agents. J. Physiol. (Lond.) 155, 13P-14P (1961)

    Google Scholar 

  • Calma, I., Wright, S.: Effects of intrathecal injection of KCl and other solutions in cats. Excitatory actions of potassium ions on posterior nerve root fibers. J. Physiol. (Lond.) 106, 211–235 (1947)

    Google Scholar 

  • Carraway, R., Leeman, S. E.: The isolation of a new hypotensive peptide, Neurotension, from bovine hypothalami. J. Biol. Chem. 248, 6854–6861 (1973)

    Google Scholar 

  • Chang, M. M., Leeman, S. E.: Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterization as substance P. J. biol. Chem. 245, 4784–4790 (1970)

    Google Scholar 

  • Cuello, A. C., Pert, J. M., Pearse, A. G. E.: Substance P: A naturally occuring transmitter in human spinal cord. Lancet 1976 II, 1054–1056 (1976)

    Google Scholar 

  • Erdös, E. G.: Enzymes that inactivate polypeptides. In: Metabolic factors controlling duration of drug actions (B. B. Brodie and E. G. Erdös, eds.), p. 159. Oxford: Pergamon Press 1962

    Google Scholar 

  • Ferreira, S. H., Moncada, S., Vane, J. R.: Prostaglandins and the mechanism of analgesia produced by aspirin-like drugs. Br. J. Pharmacol. 49, 86–97 (1973)

    Google Scholar 

  • Franz, J., Boissonnas, R. A., Stürmer, E.: Isolierung von Substanz P aus Pferdedarm und ihre biologische und chemische Abgrenzung genenüber Bradykinin. Helv. Chim. Acta 44, 881–883 (1961)

    Google Scholar 

  • Guzman, F., Braun, C., Lim, R. K. S.: Visceral pain and the pseudoaffective response to intra-arterial injection of bradykinin and other algesic agents. Arch. Int. Pharmacodyn. Ther. 136, 353–384 (1962)

    Google Scholar 

  • Henry, J. L.: Excitation of spinal nociceptive neurons by substance P. Abstr. Nobel Symp. 37, 29–31, Stockholm 1976

    Google Scholar 

  • Hökfelt, T., Kellerth, J. O., Nilsson, G., Pernow, B.: Substance P: Localization in the central nervous system and in some primary sensory neurons. Science 190, 889–890 (1975a)

    Google Scholar 

  • Hökfelt, T., Kellerth, J. O., Nilsson, G., Pernow, B.: Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons. Brain Res. 100, 235–252 (1975b)

    Google Scholar 

  • Holton, P.: Further observations on substance P in degenerating nerve. J. Physiol. (Lond.) 149, 35P-36P (1959)

    Google Scholar 

  • Iven, H., Zetler, G.: Comparison of crude bovine subcortical substance P with synthetic substance P. Naunyn-Schmiedeberg's Arch. Pharmacol. 276, 123–131 (1973)

    Google Scholar 

  • Iversen, L. L., Jessell, T., Kanazawa, I.: Release and metabolism of substance P in rat hypothalamus. Nature 264, 81–83 (1976)

    Google Scholar 

  • Jacques, R.: Discussion with G. Zetler: Naunyn-Schmiedeberg's Arch. exp. Path. Pharm. 245, 275–276 (1963)

    Google Scholar 

  • Johnson, A. R., Erdös, E.: Release of histamine from mast cells by vasoactive peptides. Proc. Soc. Exp. Biol. Med. 142, 1252–1256 (1973)

    Google Scholar 

  • Juan, H., Lembeck, F.: Action of peptides and other algesic agents on paravascular pain receptors of the isolated perfused rabbit ear. Naunyn-Schmiedeberg's Arch. Pharmacol. 283, 151–164 (1974)

    Google Scholar 

  • Juan, H., Lembeck, F.: Release of prostaglandins from the isolated perfused rabbit ear by bradykinin and acetylcholine. Agents and Actions 6, 642–645 (1976)

    Google Scholar 

  • Keele, C. A., Armstrong, D.: Substances producing pain and itch. London: Edward Arnold Ltd. 1964

    Google Scholar 

  • Lembeck, F.: Untersuchungen über die Auslösung afferenter Impulse. Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak. 230, 1–9 (1957)

    Google Scholar 

  • Lembeck, F., Starke, K.: Behandlung von Substanz P-Präparaten mit Aluminiumoxid. Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak. 259, 307–313 (1968)

    Google Scholar 

  • Lembeck, F., Juan, H.: Interaction of prostaglandins and indomethacin with algesic substances. Naunyn-Schmiedeberg's Arch. Pharmacol. 285, 301–313 (1974)

    Google Scholar 

  • Lembeck, F., Popper, H., Juan, H.: Release of prostaglandins by bradykinin as an intrinsic mechanism of its algesic effect. Naunyn-Schmiedeberg's Arch. Pharmacol. 294, 69–73 (1976)

    Google Scholar 

  • Lembeck, F., Gamse, R., Juan, H.: Substance P and sensory nerve endings. In Substance P (U. S. von Euler and B. Pernow, eds.) pp. 169–181. New York: Raven Press 1977

    Google Scholar 

  • Lim, R. K. S., Guzman, F. C.: Manifestations of pain in analgesic evaluation in animals and man. In A. Soulairac, J. Cahn and J. Charpentier: Pain, pp. 119–152. London-New York: Academic Press, 1968

    Google Scholar 

  • McGiff, J. C., Terragno, N. A., Malik, K. K., Lonigro, A. J.: Release of prostaglandin E-like substance from canine kidney by bradykinin. Circ. Res. 31, 36–43 (1972)

    Google Scholar 

  • Otsuka, M., Konishi, S.: Release of substance P-like immunoreactivity from isolated spinal cord of newborn rat. Nature 264, 83–84 (1976)

    Google Scholar 

  • Potter, G. D., Guzman, F., Lim, R. K. S.: Visceral pain evoked by intraarterial injection of substance P. Nature 193, 983–984 (1962)

    Google Scholar 

  • Strehler, B. L., Totter, J. R.: Determination of ATP and related compounds. Methods of Biochem. Analysis 1, 341–356 (1954)

    Google Scholar 

  • Studer, R. O., Trzeciak, H., Lergier, W.: Isolierung und Aminosäuresequenz von Substanz P aus Pferdedarm. Helv. Chim. Acta 56, 860–868 (1973)

    Google Scholar 

  • Stürmer, E., Berde, B.: A pharmacological comparison between synthetic bradykinin and synthetic kallidin. J. Pharmacol. exp. Ther. 139, 38–41 (1963)

    Google Scholar 

  • Takahashi, T., Otsuka, M.: Regional distribution of substance P in the spinal cord and nerve roots of the cat the effect of dorsal root section. Brain Res. 87, 1–12 (1975)

    Google Scholar 

  • Tregear, G., Niall, H. D., Potts, J. T., Leeman, S. E., Chang, M. M.: Synthesis of substance P. Nature New Biol. 232, 87–89 (1971)

    Google Scholar 

  • Zetler, G.: Zwei neue pharmakologisch aktive Polypeptide in einem substanz P-haltigen Hirnextrakt. Naunyn-Schmiedeberg's Arch. exp. Path. Pharm. 242, 330–352 (1961)

    Google Scholar 

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Lembeck, F., Gamse, R. Lack of algesic effect of substance P on paravascular pain receptors. Naunyn-Schmiedeberg's Arch. Pharmacol. 299, 295–303 (1977). https://doi.org/10.1007/BF00500324

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  • DOI: https://doi.org/10.1007/BF00500324

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