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Extensive labelling of injured insect neurons with seven different heme peptides

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Summary

Seven different heme peptides were used in neuronal uptake and labelling experiments in flies. The peptides were: catalase, lactoperoxidase, hemoglobin, horseradish peroxidase (HRP), myoglobin, cytochrome c and microperoxidase. All of these peroxidase active peptides were taken up by lesioned neurons and the markers spread throughout the entire cells resulting in a detailed labelling of their processes and cell bodies. Only HRP was taken up by intact neurons. Attempts were made to block axonal transport of HRP with colchicine, vinblastine and 2,4-dinitrophenol. These attempts were unsuccessful and it is proposed that HRP and the other six heme peptides tested are non-selectively diffusing through lesioned or damaged nerve cells in flies.

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  1. Department of Zoology, University of Lund, Lund, Sweden

    D. R. Nässel

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  1. D. R. Nässel
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Nässel, D.R. Extensive labelling of injured insect neurons with seven different heme peptides. Histochemistry 79, 95–104 (1983). https://doi.org/10.1007/BF00494346

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