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Energy linked uptake of demethylphalloin by isolated rat liver cells

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Summary

Isolated hepatocytes accumulate demethylphalloin (DMP) under aerobic conditions. In the absence of oxygen the initial rate of the DMP uptake is reduced to less than 20%, while reoxygenation restores the transport. Liver cells release previously accumulated phallotoxin when the oxygen supply is interrupted. DMP uptake is blocked by oligomycin, antimycin A, carbonylcyano-chlorophenylhydrazon (CCCP) or dinitrophenol and is partially inhibited by carboxyatractyloside. Depletion of ATP in hepatocytes by replacement of glucose by fructose reduces the accumulation of toxin too. Below 22°C no uptake was measurable. Between 22° and 37°C an apparent activation energy of 76.6 kJ/mol toxin and a Q 10 of 2.6 was calculated for the carrier mediated uptake of DMP. The results suggest that the uptake of demethylphalloin is an energy dependent substrate transfer very similar to that of cholate.

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Abbreviations

DMP:

demethylphalloin

CCCP:

carbonylcyano-chlorophenylhydrazon

DNP:

2,4-dinitrophenol

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie im Fachbereich Veterinärmedizin und Tierzucht der Justus-Liebig-Universität Giessen, Frankfurter Strasse 107, D-6300, Giessen, Federal Republic of Germany

    E. Petzinger & M. Frimmer

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  1. E. Petzinger
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  2. M. Frimmer
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Petzinger, E., Frimmer, M. Energy linked uptake of demethylphalloin by isolated rat liver cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 319, 87–92 (1982). https://doi.org/10.1007/BF00491484

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  • DOI: https://doi.org/10.1007/BF00491484

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