Abstract
The unstable catalase variant found in the blood of individuals homozygous for Swiss-type acatalasemia and the enzyme species present in heterozygous carriers of this rare defect have been further characterized. The mutant enzyme isolated from acatalasemic red cells is considerably more heat labile and differs in electrophoretic mobility from the normal enzyme. Catalase preparations obtained from heterozygotes consist of an apparently uniform enzyme species, probably representing a molecular hybrid, with properties intermediate to those of the normal and the variant enzyme. However, antigenic identity of catalase from all three sources is observed. Model experiments indicate that hybrid catalase molecules can be produced by recombining normal and variant dimer subunits. Fractionation of erythrocytes according to density and age shows that most of the residual catalase activity is localized in juvenile acatalasemic cells, whereas in normal and heterozygous individuals the catalase activity level does not alter significantly during the life span of the red cells. These findings agree with the observation that there is no gene dosage in heterozygotes, their catalase activity values falling within the normal range.
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This study is part of Project No. 3.384.74 subsidized by the Swiss National Science Foundation.
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Aebi, H., Wyss, S.R., Scherz, B. et al. Properties of erythrocyte catalase from homozygotes and heterozygotes for Swiss-type acatalasemia. Biochem Genet 14, 791–807 (1976). https://doi.org/10.1007/BF00485342
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DOI: https://doi.org/10.1007/BF00485342