Abstract
Binding of fusicoccin (FC) to microsomal preparations of corn (Zea mays L.) coleoptiles is enhanced after incubation of the tissue with indole-3-acetic acid (IAA). Treatment of the kinetic data according to Scatchard shows that the enhancement is a consequence of an increase in the number of high-affinity FC-binding sites without changes of their KD. The minimal effective concentration of IAA is 10-7 M; above 10-5 M the effect declines. The stimulation is insensitive to protein-synthesis inhibitors (cycloheximide and puromycin). The same effect is observed with the synthetic auxins 2,4-dichlorophenoxyacetic acid and naphtalene-1-acetic acid while it is abolished by the auxin antagonists naphtalene-2-acetic acid and p-chlorophenoxyisobutyric acid. Since the above effect is only observed with intact tissue and not after incubation of IAA with microsomal preparations, a direct interaction of IAA with the FC-binding sites is ruled out and an alternative mechanism must be sought.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- FC:
-
fusicoccin
- [3H]FC:
-
3H-labeled dihydrofusicoccin
- IAA:
-
indole-3-acetic acid
- 1-NAA:
-
naphtalene-1-acetic acid
- 2-NAA:
-
naphtalene-2-acetic acid
- PCIB:
-
p-chlorophenoxyisobutyric acid
References
Aducci, P., Ballio, A., Federico, R., Scalorbi, D. (1979) Enhancement of fusicoccin binding to corn coleoptiles plasma membranes by in vivo treatment with indole-3-acetic acid. G. Bot. Ital. 113, 191
Aducci, P., Ballio, A., Fiorucci, L., Simonetti, E. (1984b) Inactivation of solubilised fusicoccin-binding sites by endogenous plant hydrolases. Planta 160, 422–427
Aducci, P., Coletta, M., Marra, M. (1984a) An improved Scatchard analysis of fusicoccin-binding to maize coleoptile membranes. Plant Sci. Lett. 33, 187–193
Aducci, P., Crosetti, G., Federico, R., Ballio, A. (1980) Fusicoccin receptors. Evidence for endogenous ligand. Planta 148, 208–210
Ballio, A., Carilli, A., Santurbano, B., Tuttobello, L. (1968) Produzione di fusicoccina in scala pilota. Ann. Ist. Sup. Sanità 4, 317–322
Ballio, A., Federico, R., Pessi, A., Scalorbi, D. (1980) Fusicoccin binding sites in subcellular preparations of spinach leaves. Plant Sci. Lett. 18, 39–44
Ballio, A., Federico, R., Scalorbi, D. (1981) Fusicoccin structure-activity relationships: in vitro binding to microsomal preparations of maize coleoptiles. Physiol. Plant. 52, 476–481
Buckhout, T.J., Young, K.A., Low, P.S., Morré, D.J. (1981) In vitro promotion by auxins of divalent ion release from soybean membranes. Plant Physiol. 68, 512–515
Cleland, R.E. (1976) Fusicoccin induced growth and hydrogen ion excretion of Avena coleoptiles: relation to auxin respones. Planta 128, 201–206
Dohrmann, U., Pesci, P., Cocucci, S.M., Marrè, E. (1977) Stimulating effect of fusicoccin on K+-activated ATPase in plasmalemma preparations from higher plant tissues. Plant Sci. Lett. 8, 91–98
Hertel, R. (1983) The mechanism of auxin transport as a model for auxin action. Z. Pflanzenphysiol 112, 53–67
Jacobs, M., Hertel, R. (1978) Auxin binding to subcellular fractions from Cucurbita hypocotyls: in vitro evidence for an auxin transport carrier. Planta 142, 1–10
Kutschera, U., Schopfer, P. (1985a) Evidence against the acid-growth theory of auxin action. Planta 163, 483–493
Kutschera, U., Schopfer, P. (1985b) Evidence for the acid-growth theory of fusicoccin action. Planta 163, 494–499
Lado, P., Pennacchioni, A., Rasi Caldogno, F., Russi, S., Silano, V. (1972) Comparison between some effects of fusicoccin and indole-3-acetic acid on cell enlargement in various plant materials. Physiol. Plant Pathol. 2, 75–85
Lees, M.B., Paxman, S. (1972) Modification of the Lowry procedure for the analysis of proteolipid protein. Anal. Biochem. 47, 184–192
Marmé, D., Dieter, P. (1983) The role of Ca2+ and calmodulin in plants. In: Calcium and cell function, vol. 4, pp. 263–311, Cheung, W.Y., ed. Academic Press, New York London
Marrè, D. (1979) Fusicoccin, a tool in plant physiology. Annu. Rev. Plant Physiol. 30, 273–288
Rubinstein, B., Cleland, R.E. (1981) Responses of Avena coleoptiles to suboptimal fusicoccin: kinetics and comparisons with indoleacetic acid. Plant Physiol. 68, 543–547
Scatchard, G. (1949) The attractions of proteins for small molecules. Ann. N.Y. Acad. Sci. 51, 660–672
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aducci, P., Ballio, A. & Marra, M. Incubation of corn coleoptiles with auxin enhances in-vitro fusicoccin binding. Planta 167, 129–132 (1986). https://doi.org/10.1007/BF00446379
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00446379