Summary
An in vitro system for replication of mini-F plasmid DNA was constructed. This system consists of an ammonium sulfate fraction II (Fuller et al. 1981) from Escherichia coli extract, exogeneously added purified E protein encoded by mini-F plasmid, and mini-F DNA in a closed circular form. Experiments with this system showed that the 217 bp DNA region which contains the A+T rich cluster and the four 19 bp direct repeats responsible for incB incompatibility is essential for mini-F DNA replication.
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References
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Communicated by M. Takanami
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Muraiso, K., Tokino, T., Murotsu, T. et al. Replication of mini-F plasmid in vitro promoted by purified E protein. Mol Gen Genet 206, 519–521 (1987). https://doi.org/10.1007/BF00428895
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DOI: https://doi.org/10.1007/BF00428895