Summary
A new linear DNA plasmid, designated pSKL, was found in the yeast Saccharomyces kluyveri. Restriction maps of the 14.2 kb plasmid were constructed. By the use of CsCl-Hoechst 33258 centrifugation containing guanidine chloride, pSKL was isolated as a DNA-protein complex. The protein was associated with the terminal regions of pSKL. The two terminal EcoRI fragments of pSKL were cloned and their nucleotide sequences were determined. pSKL had inverted terminal repeats of 483 bp with a unique structure in which fairly homologous sequences of 30 bp were repeated eight times.
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References
Carusi EA (1977) Evidence for blocked termini in human adenovirus DNA. Virology 76:380–394
Challberg MD, Kelly TJ (1982) Eucaryotic DNA replication: viral and plasmid model systems. Annu Rev Biochem 51:901–934
Erickson L, Beversdorf WD, Pauls KP (1985) Linear mitochondrial plasmid in Brassica has terminal protein. Curr Genet 9:679–682
Escarmis C, Salas M (1981) Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage ϕ29. Proc Natl Acad Sci USA 78:1446–1450
Escarmis C, Gomez A, Garcia E, Ronda C, Lopez R, Salas M (1984) Nucleotide sequences at the termini of the DNA of Streptococcus pneumoniae phage Cp-1. Virology 133:166–171
Escarmis C, Garcia P, Mendes E, Lopez R, Salas M, Garcia E (1985) Inverted terminal repeats and terminal proteins of the genomes of pneumococcal phages. Gene 36:341–348
Garcia E, Gomez A, Ronda C, Escarmis C, Lopez R (1983) Pneumococcal bacteriophage Cp-1 contains a protein bound to the 5′ termini of its DNA. Virology 128:92–104
Gunge N, Tamaru A, Ozawa F, Sakaguchi K (1981) Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J Bacteriol 145:382–390
Harding NE, Ito J, David GS (1978) Identification of the protein firmly bound to the ends of bacteriophage ϕ29 DNA. Virology 84:279–292
Hayakawa T, Tanaka T, Sakaguchi K, Otake N, Yonehara H (1979) A linear plasmid-like DNA in Streptomyces sp. producing lankacidin group antibiotics. J Gen Appl Microbiol 25:225–260
Hirochika H, Nakamura K, Sakaguchi K (1984) A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs. EMBO J 3:761–766
Hishinuma F, Nakamura K, Hirai K, Nishizawa R, Gunge N, Maeda T (1984) Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast. Nucleic Acids Res 12:7581–7597
Ito J (1978) Bacteriophage ϕ29 terminal protein: its association with 5′-termini of the ϕ29 genome. J Virol 28:895–904
Kemble RJ, Thompson D (1982) S1 and S2, the linear mitochondrial DNAs present in a male sterile line of maize, possess terminally attached proteins. Nucleic Acids Res 10:8181–8190
Kikuchi Y, Hirai K, Hishinuma F (1984) The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins. Nucleic Acids Res 12:5685–5692
Levings III CS, Sederoff RR (1983) Nucleotide sequence of the S-2 mitochondrial DNA from the S cytoplasm of maize. Proc Natl Acad Sci USA 80:4055–4059
Paillard M, Sederoff RR, Levings III CS (1985) Nucleotide sequence of the S-1 mitochondrial DNA from the S cytoplasm of maize. EMBO J 4:1125–1128
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) An unusual mitochondrial DNA plasmid in the genus Brassica. Nature 301:725–728
Pring DR, Levings III CS, Hu WWL, Timothy DH (1977) Unique DNA associated with mitochondria in the “S”-type cytoplasm of male-sterile maize. Proc Natl Acad Sci USA 74:2904–2908
Rekosh DMK, Russel WC, Bellett AJD, Robinson AJ (1977) Identification of a protein linked to the ends of adenovirus DNA. Cell 11:283–295
Salas M, Mellado RP, Vinuela E, Sogo JM (1978) Characterization of a protein covalently linked to the 5′ termini of the DNA of Bacillus subtilis phage ϕ 29. J Mol Biol 119:269–291
Salas M (1983) A new mechanism for the initiation of replication of ϕ29 and adenovirus DNA: priming by the terminal protein. Curr Top Microbiol Immunol 109:89–106
Sanger F, Nicklen S, Coulson AR(1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Stark MJR, Mileham AJ, Romanos MA, Boyd A (1984) Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis. Nucleic Acids Res 12:6011–6030
Sor F, Wesolowski M, Fukuhara H (1983) Inverted terminal repetitions of the two linear DNA associated with the killer character of the yeast Kluyveromyces lactis. Nucleic Acids Res 11:5037–5044
Tolun A, Alestrom P, Pettersson U (1979) Sequence of inverted terminal repetitions from different adenoviruses: demonstration of conserved sequences and homology between SA7 termini and SV40 DNA. Cell 17:705–713
Tu CPD, Cohen SN (1980) 3′-end labeling of DNA with (α-32P) cordycepin-5′-triphosphate. Gene 10:177–183
Wallace RB, Johnson MJ, Suggs SV, Miyoshi K, Bhatt R, Itakura K (1981) A set of synthetic oligodeoxy ribonucleotide primers for DNA sequencing in the plasmid vector pBR322. Gene 16:21–26
Yoshikawa H, Ito J (1981) Terminal proteins and short inverted terminal repeats of the small Bacillus bacteriophage genome. Proc Natl Acad Sci USA 78:2596–2600
Yoshikawa H, Friedman T, Ito J (1981) Nucleotide sequences at the termini of ϕ29 DNA. Proc Natl Acad Sci USA 78:1336–1340
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Communicated by C.R. Hollenberg
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Kitada, K., Hishinuma, F. A new linear DNA plasmid isolated from the yeast Saccharomyces kluyveri . Mol Gen Genet 206, 377–381 (1987). https://doi.org/10.1007/BF00428874
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DOI: https://doi.org/10.1007/BF00428874