Summary
Spontaneous chloramphenicol-sensitive mutants of Streptomyces lividans 66 had previously been shown to be very unstable and to yield arginine auxotrophic mutants at a frequency of 25% of spores; the Arg- mutants had amplified a particular 5.7 kb DNA sequence to over one hundred tandem copies per genome. In this paper we report the cloning of the amplifiable region from amplified and wild-type strains. This showed that the amplifiable fragment is already present as a duplication in wild type cells. Hybridisation experiments also demonstrated that in the amplified strains there was a deletion of neighbouring DNA sequences to one side of the amplifiable element; sequences to the other side remain intact.
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Communicated by H. Saedler
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Altenbuchner, J., Cullum, J. Structure of an amplifiable DNA sequence in Streptomyces lividans 66. Molec. Gen. Genet. 201, 192–197 (1985). https://doi.org/10.1007/BF00425659
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DOI: https://doi.org/10.1007/BF00425659