Abstract
Lipopolysaccharides (LPS), added as whole bacteria to estuarine sediments, were extracted efficiently by both trichloroacetic acid (TCA) and phenol-water (PW). Amounts of recovered LPS were measured indirectly by analyses for ketodeoxyoctonate (KDO), β-hydroxymyristic acid, immunodominant sugars and anticomplementary (AC) activity towards human complement. TCA was judged to be better than PW for routine extraction of sediments because, although it yielded 10–20% less LPS, it avoided contamination with non-LPS, high-molecular weight material with high AC activity. In sediment samples taken as cores from estuarine beaches, the concentration of endogenous LPS diminished rapidly with depth below the topmost 1 cm. KDO disappeared more rapidly with depth than AC activity. When known LPS was incubated with estuarine beach mud at 20–22°C for 3 weeks there was extensive biodegradation of both the lipid and polysaccharide components, the latter more rapidly. LPS-degrading bacteria were isolated.
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Boivin, A., Mesrobeanu, J. et Mesrobeanu, L. 1933. Technique pour la préparation des polyosides microbiens specifiques. — Compt. Rend. Soc. Biol. 113: 490–492.
Ellwood, D. C. 1970. The distribution of 2-keto-3-deoxyoctonic acid in bacterial walls. —J. Gen. Microbiol. 60: 373–381.
Evans, T. M., Schillinger, J. E. and Stuart, D. G. 1978. Rapid determination of bacteriological water quality by using Limulus lysate. — Appl. Environ. Microbiol. 35: 376–382.
Galanos, C., Rietschel, E. Th., Lüderitz, O. and Westphal, O. 1971. Interaction of lipopolysaccharides and lipid A with complement. — Eur. J. Biochem. 19: 143–152.
Galanos, C., and Lüderitz, O. 1976. The role of the physical state of lipopolysaccharide in the interaction with complement. — Eur. J. Biochem. 65: 403–408.
Inai, S., Kazuyoshi, N., Ebisu, S., Keÿino, K., Kotani, S. and Nisaki, A. 1976. Activation of the alternative complement pathway by water insoluble glucans of Streptococcus mutans; the relation between their chemical structures and activating potencies. — J. Immunol. 117: 1256–1260.
Jorgensen, J. M., Lee, J. C. and Pahren, H. R. 1976. Rapid detection of bacterial endotoxins in drinking water and renovated waste water. — Appl. Environ. Microbiol. 32: 347–351.
Kates, M. 1972. Isolation, analysis and identification of lipids. p. 446–469. In: Techniques in lipidology. — North Holland Press, Amsterdam.
Keleti, G. and Lederer, W. E. 1974. Handbook of micromethods for the biological sciences. — Van Nostrand Reinhold, New York.
Neter, E. 1956. Bacterial haemagglutination and haemolysis. — Bacteriol. Rev. 20: 166–171.
Rheinheimer, G. 1974. p. 35. In: Aquatic microbiology — John Wiley and Sons, New York.
Rietschel, E. Th., Galanos, C. and Lüderitz, O. 1975. Structure, endotoxicity, immunogenicity of the lipid A component of bacterial lipopolysaccharides. p. 307–314. In D. Schlessinger (ed). Microbiology. — American Society for Microbiology, Washington.
Saddler, J. N. and Wardlaw, A. C. 1978. Superiority of human complement for assaying bacterial lipopolysaccharides by their anticomplementary activity. — Experientia 34: 1227–1228.
Thatcher, R. C. and Weaver, T. L. 1974. Simplified method for the preparation of silica media. — Appl. Microbiol. 28: 887–888.
Voets, J. P., Vandamme, E. J. and De Maerteleine, E. 1973. Biodegradation of microbial lipopolysaccharides. — Experientia 29: 730–731.
Waravdekar, V. S. and Saslaw, L. D. 1959. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. — J. Biol. Chen. 234: 1945–1950.
Westphal, O., Lüderitz, O. und Bister, F. 1952. Über bakterielle Reizstoffe 1. mit Reindarstellung eines Polysaccharid-Pyrogens aus Bacterium coli. — Z. Naturforsch. 7b: 537–548.
Winkelstein, J. A. and Tomasz, A. 1978. Activation of the alternative complement pathway by Pneumococcal cell wall teichoic acid. — J. Immunol. 120: 174–178.
ZoBell, C. E. and Upham, H. C. 1944. A list of marine bacteria including descriptions of sixty new species. — Bull. Scripps Inst. Oceanogr. 5: 239–292.
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Saddler, J.N., Wardlaw, A.C. Extraction, distribution and biodegradation of bacterial lipopolysaccharides in estuarine sediments. Antonie van Leeuwenhoek 46, 27–39 (1980). https://doi.org/10.1007/BF00422226
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DOI: https://doi.org/10.1007/BF00422226