Abstract
Rice bran was superior to other proteinaceous substrates for protease production by Rhizopus oligosporus ACM 145F in solid-state fermentation. Maximum protease yield was after 72 h. The optimal initial moisture content was 47% (a w=0.97). Dried, ground and resuspended fermented rice was the most pratical and effective inoculum preparation, although, in the laboratory, spore suspensions prepared directly from agar slants were more convenient. Inoculum density (from 102 to 107 spores/g substrate) and age (3, 5, 7 and 9 days) had little effect on protease yield.
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The authors are with the Department of Chemical Engineering, The University of Queensland, Brisbane, Queensland, 4072, Australia
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Ikasari, L., Mitchell, D.A. Protease production by Rhizopus oligosporus in solid-state fermentation. World Journal of Microbiology & Biotechnology 10, 320–324 (1994). https://doi.org/10.1007/BF00414872
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DOI: https://doi.org/10.1007/BF00414872