Abstract
Low concentration of dissolved O2 (3 μM) induced a significant C2H2 reduction by bacteriods isolated from French-beans (Phaseolus vulgaris) inoculated with Rhizobium phaseoli strain 9-6. This activity, determined with or without glucose, declined with increasing O2 concentrations in spite of the generation of a substantial level of ATP. Under these conditions, cytochrome c studied by a rapid spectrometry method on whole cells, appeared more than 80% oxidized. An active C2H2 reduction always corresponded to a high level of cyt c reduction observed with glucose, at low O2 tensions only, and with succinate for a larger range of pO2 values. Our results indicated that inability of glucose to support C2H2 reduction activity for higher O2 tensions was due to a limiting electron allocation to nitrogenase in relation with the poor level of reduced cyt c observed in these conditions.
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Trinchant, J.C., Birot, A.M., Denis, M. et al. C2H2 reduction, oxygen uptake and cytochrome c reduction by bacteroids isolated from French-bean nodules. Arch. Microbiol. 134, 182–186 (1983). https://doi.org/10.1007/BF00407755
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DOI: https://doi.org/10.1007/BF00407755