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On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae

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Abstract

Mutants of Escherichia coli were isolated in which transcription of the structural genes for hydrogenase (hyd) and for one of the components of formate dehydrogenase (fdh) (of the formate hydrogen-lyase complex) is coupled with that of the lacZ gene. They were — together with lac fusions of the nifH and nifL genes from Klebsiella — used to study regulation by redox control, of the expression of the respective structural genes. The following results were obtained: (i) β-galactosidase synthesis was fully repressed in the presence of O2 or nitrate (anaerobically), and induced in the absence of an external electron acceptor. Fumarate as terminal electron acceptor only marginally affected nif expression and partially repressed hyd and fdh expression. Redox control of the synthesis of hydrogenase and formate dehydrogenase, therefore, (as well as that of nif) acts at the level of transcription; the size of the redox potential seems to be correlated with the amount of repression; (ii) β-galactosidase synthesis in the hyd:: lac and fdh::lac fusion strains is induced by formate. At high concentrations formate reverses the repression by nitrate and fumarate but not that by oxygen.

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Author notes

  1. H. Hennecke

    Present address: Mikrobiologisches Institut, ETH Zürich, Universitätsstrasse 2, CH-8092, Zürich, Switzerland

Authors and Affiliations

  1. Lehrstuhl für Mikrobiologie der Universität München, Maria-Ward-Straße 1 a, D-8000, München 19, Federal Republic of Germany

    A. Pecher, F. Zinoni, C. Jatisatienr, R. Wirth, H. Hennecke & A. Böck

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  1. A. Pecher
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  2. F. Zinoni
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  3. C. Jatisatienr
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  4. R. Wirth
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  5. H. Hennecke
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  6. A. Böck
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Pecher, A., Zinoni, F., Jatisatienr, C. et al. On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae. Arch. Microbiol. 136, 131–136 (1983). https://doi.org/10.1007/BF00404787

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  • DOI: https://doi.org/10.1007/BF00404787

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