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Influence of intercellular agents on proliferation and gene activity of cultured human skin epithelium cells (NCTC 2544)

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Summary

“Human skin epithelium cells” (NCTC strain 2544; HSEpicell) are established cells and grow to a monolayer the same way as epithelial cells. Addition of proliferating or antiproliferating substances results in a typical dose-dependent influence on the cell growth: steroids inhibit mitosis, polyamines stimulate proliferation, while prostaglandin E2, theophylline and papaverine reduce cell growth.

Since the pattern of chromosomal nonhistone proteins indicates alterations of gene activity, DNA-binding proteins of HSEpicells are analysed. Compared to native human skin fibroblasts (La Col 1115) there are only slight differences, in contrast to cancer cells. Therefore HSEpicells may represent undifferentiated non-cancer cells. Hydrocortisone and theophylline inhibit cell proliferation by different mechanisms. As indicated by the pattern of DNA-binding proteins, both substances also act on HSEpicells in two different ways.

As HSEpicells can be used for studying cell regulation, water-soluble extract and DNA-binding proteins of psoriatic scales as well as sera of psoriasis patients are tested in respect to any proliferating component. However, no influence on cell proliferation could be found.

Zusammenfassung

“Human skin epithelium cells” (NCTC strain 2544; HuSki-Epicell) ist ein permanenter Zellstamm, der im Monolayer wie Epithelzellen Desmosomen ausbildet. Exogene Zugabe von proliferativ bzw. antiproliferativ wirkenden Zellregulatoren beeinflußt dosisabhängig das Wachstum der Zellkultur in typischer Weise: Steroide hemmen die Zellteilung, Polyamine die Proliferation, während Prostaglandin E2, Theophyllin und Papaverin das Wachstum bremsen.

Da das Muster chromosomaler Nicht-Histon-Proteine die Veränderungen von Genaktivitäten anzeigen, wurden DNA-affine Proteine der HuSkiEpicell vergleichend studiert. Gegenüber nativen menschlichen Hautfibroblasten (LaCol 1115) bestehen im Gegensatz zu cancerogen veränderten Zellen lediglich geringe Unterschiede; somit dürften die HuSkiEpicell jugendliche, nicht cancerogen veränderte Zellen darstellen. Hydrokortison und Theophyllin bewirken über verschiedene Mechanismen eine Hemmung der Zellproliferation. Dies trifft auch bei HuSkiEpicell zu, wie Veränderungen im Muster DNA-affiner Proteine andeuten.

Da die HuSkiEpicell somit zum Studium von Zellregulationen geeignet erscheint, wurden wasserlöslicher Extrakt und DNA-affine Proteine aus Psoriasisschuppen sowie Psoriatiker-Serum auf proliferative Komponenten untersucht. Es konnte jedoch weder im Vollmedium noch im Minimalmedium ein signifikanter Unterschied im Wachstum der HuSkiEpicell gefunden werden.

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Authors and Affiliations

  1. Center of Dermatology and Venereology, Department IV (Biochemistry), University of Frankfurt, D-6000, Frankfurt am Main, Federal Republic of Germany

    G. Reimer, M. Mentzer, K. Gottschalk & A. Neufahrt

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  1. G. Reimer
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  2. M. Mentzer
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  3. K. Gottschalk
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  4. A. Neufahrt
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Dedicated to Professor Dr. G. Leonhardi on the occasion of his 65th birthday

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Reimer, G., Mentzer, M., Gottschalk, K. et al. Influence of intercellular agents on proliferation and gene activity of cultured human skin epithelium cells (NCTC 2544). Arch Dermatol Res 270, 313–324 (1981). https://doi.org/10.1007/BF00403935

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  • DOI: https://doi.org/10.1007/BF00403935

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