Abstract
Quantitative cytochemical studies of cortical parenchyma cells of roots of Pisum sativum in which the central vascular bundle is severed, showed esterase activity to be an early marker of the determination of cells to form a vascular bridge. Explantation, onto a basal culture medium, of wound segments taken from roots at different times after severing the stele showed the irreversibility of the esterase activity on removal from the inducing environment, so confirming this as a marker of cell determination. A general determination for the stele was shown to occur by 8–10 h after wounding, but information relating to tracheid secondary-cell-wall formation was not apparently available until 18–20 h after wounding. Determination appeared to occur well before mitosis. The timings of the differentiation steps indicate a simple diffusion model to explain the mechanism of arrival of the initiating molecules.
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References
Altman, F.P. (1980) How thick is your section? (Abstr.) 6th Int. Cong. Histochem., Brighton, p. 6
Bonnemain, J.L. (1974) Transport et distribution des traceurs après application de AIA-2-14C sur les feuilles de Vicia faba. C.R. Acad. Sci. Paris 273, 1699–1702
Butcher, R.G. (1971) The chemical determination of section thickness. Histochemie 28, 131–136
Dodds, J.H. (1981) The role of the cell cycle and cell division in xylem differentiation. In: Aspects of xylem cell development, pp. 153–166, Barnett, J.R. ed. Castle House Publ., Tunbridge Wells
Gahan, P.B. (1972) The role of acid hydrolases in the differentiation of plant cells. In: Cell differentiation, pp. 223–224, Harris, R., Allin, P., Viza, D., eds. Munksgaard, Copenhagen
Gahan, P.B. (1981a) Biochemical changes during xylem element differentiation. In: Aspects of xylem cell development, Barnett, J.R., ed. Castle House Publ., Tunbridge Wells
Gahan, P.B. (1981b) An early cytochemical marker of commitment to stelar differentiation in meristems from dicotyledonous plants. Ann. Bot. 48, 769–775
Gahan, P.B., McLean, J., Kalina, M., Sharma, W. (1967) Freeze-sectioning of plant tissues: the technique and its use in plant histochemistry. J. Exp. Bot. 18, 151–159
Jacobs, W.P. (1979) Plant hormones and plant development. Cambridge University Press, Cambridge
McLean, J., Gahan, P.B. (1970) The distribution of acid phosphatases and esterases in differentiating roots of Vicia faba. Histochemie 24, 41–49
Meins, F., Jr., Binns, A.N. (1979) Cell determination in plant development. Bio Science 29, 221–225
Mitchison, G.J. (1981) The polar transport of auxin and vein patterns in plants. Philos. Trans. R. Soc. London Ser. B 295, 461–472
Morris, D.A., Kadir, G.O. (1972) Pathway of auxin transport in intact pea seedlings. Planta 107, 171–182
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Phillips, R., Dodds, J.H. (1977) Rapid differentiation of tracheary elements in cultured explants of Jerusalem artichoke. Planta 135, 207–212
Phillips, R., Torrey, J.G. (1973) DNA synthesis, cell division and specific cytodifferentiation in cultured pea root cortical explants. Dev. Biol. 31, 336–347
Rana, M.A. (1982) Cytochemical studies of cell determination and differentiation in dicotyledonous plants with special reference to the stelar tissue. Ph.D. thesis, University of London
Rana, M.A., Gahan, P.B. (1982) Initiation of determination of stele elements in roots of Pisum sativum. Ann. Bot. (London) 50, 757–762
Robbertse, P.J., McCully, M.E. (1979) Regeneration of vascular tissue in wounded pea roots. Planta 145, 167–173
Salisbury, F.B., Ross, C. (1969) Plant physiology, pp. 167–187. Wadsworth Publ. Co., Belmont, Cal.
Shininger, T.L. (1980) Biochemical and cytological analysis of RNA synthesis in kinetin-treated pea root parenchyma. Plant Physiol. 65, 838–843
Shininger, T.L., Polley, L.D. (1977) Biochemical responses of pea root tissue to cytokinin: enhanced rate of RNA synthesis. Plant Physiol. 59, 831–835
Simpson, S.F., Torrey, T.G. (1977) Hormonal control of deoxyribonucleic acid and protein synthesis in pea root cortical explants. Plant Physiol. 59, 4–9
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Rana, M.A., Gahan, P.B. A quantitative cytochemical study of determination for xylem-element formation in response to wounding in roots of Pisum sativum L.. Planta 157, 307–316 (1983). https://doi.org/10.1007/BF00397401
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DOI: https://doi.org/10.1007/BF00397401