Abstract
Previously, we have shown that the monomeric-sugar composition of cell-surface-associated glycoconjugates of two strains of Chlamydomonas eugametos, of different mating type, differs strikingly (Gerwig et al. 1984, Carbohydr. Res. 127, 245–251). Besides the common occurrence of various pentoses and hexoses, the glycoconjugates of one strain contain 4-O-methyl xylose, a 2-O-methyl pentose (probably 2-O-methyl arabinose) and 3-O-methyl galactose, whereas those of the other strain contain 6-O-methyl mannose and 3-O-methyl glucose. In order to investigate whether these differences are relevant to the mating process of this organism, the sugar composition of the sexual progeny of these strains was analyzed. The ability to produce 4-O-methyl xylose, 2-O-methyl pentose and 3-O-methyl galactose on the one hand, and the ability to produce 6-O-methyl mannose and 3-O-methyl glucose on the other hand, appear to be genetically linked. However, the ability to produce either set of O-methyl sugars was inherited independently of mating type. O-Methylated sugars do not occur in the cell wall of C. eugametos, or in the cell-free medium, but only in surface-membrane-associated glycoconjugates, extractable with salt or detergent solutions.
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Abbreviations
- mt +/- :
-
mating-type plus or minus
References
Adair, W.S., Hwang, C., Goodenough, U.W. (1983) Identification and visualization of the sexual agglutinin from the mating type plus flagellar membrane of Chlamydomonas. Cell 33, 183–193
Colin-Osdoby, P., Adair, S.W. (1985) Characterization of the purified Chlamydomonas minus agglutinin. J. Cell Biol. 101, 1144–1152
Gerwig, G.J., Kamerling, J.P., Vliegenthart, J.F.G., Homan, W.L., van Egmond, P., van den Ende, H. (1984) Characteristic differences in monosaccharide composition of glycoconjugates from opposite mating types of Chlamydomonas eugametos. Carbohydr. Res. 127, 245–251
Homan, W.L., van Kalshoven, H., Kolk, A.H.J., Musgrave, A., Schuring, F., van den Ende, H. (1986) Monoclonal antibodies to surface glycoconjugates in Chlamydomonas eugametos recognize strain-specific ±O-methyl sugars. Planta 170, 328–335
Klis, F.M., Samson, M.R., Touw, E., Musgrave, A., van den Ende, H. (1985) Sexual agglutination in the unicellular green alga Chlamydomonas eugametos. Plant Physiol. 79, 740–745
Lechner, J., Wieland, F., Sumper, M. (1985) Transient methylation of dolichyl oligosaccharides is an obligatory step in halobacterial sulfated glycoprotein biosynthesis. J. Biol. Chem. 260, 8984–8989
Lewin, R.A. (1952) Studies on the flagella of algae. I. General observation on Chlamydomonas moewusii Gerloff. Biol. Bull. (Woods Hole, Mass.) 102, 74–79
Lewin, R.A. (1953) The genetics of Chlamydomonas moewusii Gerloff. J. Genet. 51, 543–560
Matsuda, Y., Sakamoto, K., Kiuchi, N., Mizuochi, T., Tsubo, Y., Kobata, A. (1982) Two tunicamycin-sensitive components involved in agglutination and fusion of Chlamydomonas reinhardtii gametes. Arch. Microbiol. 131, 87–90
Mesland, D.A.M. (1976) Mating in Chlamydomonas eugametos. A scanning electron microscopical study. Arch. Microbiol. 109, 31–35
Musgrave, A., van Eijk, E., te Welscher, R., Broekman, R., Lens, P.F., Homan, W.L., van den Ende, H. (1981) Sexual agglutination factor from Chlamydomonas eugametos. Planta 153, 362–369
Samson, M.R., Klis, F.M., Homan, W.L., van Egmond, P., Musgrave, A., van den Ende, H. (1987) Composition and properties of the sexual agglutinins of the flagellated green alga Chlamydomonas eugametos. Planta, in press
Spiro, R.G. (1972) Study of the carbohydrates of glycoproteins. Methods Enzymol. 28, 3–43
Tomson, A.M., Demets, R., Bakker, N.P.M., Stegwee, D., van den Ende, H. (1985) Gametogenesis in liquid cultures of Chlamydomonas eugametos. J. Gen. Microbiol. 131, 1553–1560
van den Ende, H. (1985) Sexual agglutination in the Chlamydomonads. Adv. Microb. Physiol. 26, 89–123
Wiese, L., Mayer, R.A. (1982) Unilateral tunicamycin sensitivity of gametogenesis in dioecious isogamous Chlamydomonas species. Gamete Res. 5, 1–9
Wiese, L., Wiese, W. (1975) On sexual agglutination and mating type substances in isogamous dioecious chlamydomonads. IV. Unilateral inactivation of the sex contact capacity in compatible and incompatible taxa by α-mannosidase and snake venom protease. Dev. Biol. 43, 264–276
Witman, G.B., Carlson, K., Berliner, J., Rosenbaum, J.L. (1972) Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes and mastigonemes. J. Cell Biol. 54, 507–539
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Schuring, F., Smeenk, J.W., Homan, W.L. et al. Occurrence of O-methylated sugars in surface glycoconjugates in Chlamydomonas eugametos . Planta 170, 322–327 (1987). https://doi.org/10.1007/BF00395023
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DOI: https://doi.org/10.1007/BF00395023