Abstract
The aim of this work was to describe the photosynthetic carbon metabolism of the cooltemperate C4 grass Spartina anglica. With the exception of pyruvate, phosphate dikinase and pyruvate kinase, the maximum catalytic activities in leaves of putative enzymes of the C4 cycle of a phosphoenolpyruvate-carboxykinase C4 plant were considerably in excess of the observed, steady-state rate of photosynthesis, and were comparable with the maximum catalytic activities of key enzymes of the reductive pentose-phosphate pathway. Radioactive carbon from 14CO2 supplied to attached leaves during steady-state photosynthesis appeared first in malate and aspartate from which it moved to intermediates of the reductive pentose-phosphate pathway, and then to sucrose. These experiments show that photosynthetic carbon metabolism in this cool-temperate C4 plant is similar to that of C4 plants of hotter climates.
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Andrews, T.J., Johnson, H.S., Slack, C.R., Hatch, M.D. (1971) Malic enzyme and aminotransferases in relation to 3-phosphoglycerate formation in plants with the C4-dicarboxylic acid pathway of photosynthesis. Phytochemistry 10, 2005–2013
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Bassham, J.A., Calvin, M. (1957) The path of carbon in photosynthesis. Prentice-Hall, Englewood Cliffs, N.J.
Berry, J., Bjorkman, O. (1980) Photosynthetic response and adaptation to temperature in higher plants. Annu. Rev. Plant Physiol. 31, 491–543
Caldwell, M.M., Osmond, C.B., Nott, D.L. (1977) C4 pathway photosynthesis at low temperature in cold-tolerant Atriplex species. Plant Physiol. 60, 1571–164
Dick, P.S., ap Rees, T. (1975) The pathway of sugar transport in roots of Pisum sativum. J. Exp. Bot. 26, 305–314
Edwards, G.E., Huber, S.C., Ku, S.B., Rathnam, C.K.M., Gutierrez, M., Mayne, B.C. (1975) Variation in photochemical activities of C4 plants in relation to CO2 fixation. In: CO2 metabolism and plant productivity, pp. 83–112, Burris, R.H., Black, C.C., eds. University Park Press, Baltimore, Md.
Evans, J.H. (1963) Effect of potassium and other univalent cations on activity of pyruvate kinase in Pisum sativum. Plant Physiol. 38, 397–402
Harley, J.L., Beevers, H. (1963) Acetate utilization by maize roots. Plant Physiol. 38, 117–123
Hatch, M.D. (1979) Mechanism of C4 photosynthesis in Chloris gayana: pool sizes and kinetics of 14CO2 incorporation into 4-carbon and 3-carbon intermediates.Arch. Biochem. Biophys. 194, 117–127
Hatch, M.D., Kagawa, T. (1974) NAD-malic enzyme in leaves with C4-pathway photosynthesis and its role in C4 acid decarboxylation. Arch. Biochem. Biophys.160, 346–349
Hatch, M.D., Kagawa, T. (1976) Photosynthetic activities of isolated bundle sheath cells in relation to differing mechanisms of C4 pathway photosynthesis. Arch. Biochem. Biophys. 175, 39–53
Hatch, M.D., Slack, C.R. (1969) NADP-specific malate dehydrogenase and glycerate kinase in leaves and evidence for their location in chloroplasts. Biochem. Biophys. Res. Commun. 34, 589–593
Hatch, M.D., Slack, C.R. (1975) Pyruvate, Pi dikinase from leaves. Methods Enzymol 42, 212–219
Hatch, M.D., Tsuzuki, M., Edwards, G.E. (1982) Determination of NAD malic enzyme in leaves of C4 plants. Plant Physiol.69, 483–491
Ireland, R.J., DeLuca, V., Dennis, D.T. (1979) Isoenzymes of pyruvate kinase in etioplasts and chloroplasts. Plant Physiol. 63, 903–907
Johnson, H.S., Hatch M.D.(1969) The C4-dicarboxylic acid pathway of photosynthesis. Identification of intermediates and products and quantitative evidence for the roure of carbon flow. Biochem. J. 114, 127–134
Johnson, H.S., Hatch, M.D. (1970) Properties and regulation of leaf nicotinamide-adenine dinucleotide phosphate-malate dehydrogenase and “malic” enzyme in plants with the C4-dicarboxylic acid pathway of photosynthesis. Biochem. J. 119, 273–280
Jones, D.A., Smith, A.M., Woolhouse, H.W. (1983) An apparatus for pulse and pulse-chase experiments with 14CO2 on attached leaves with known, steady-state rates of photosynthesis. Plant Cell Environ. (in press)
Layne, E. (1957) Spectrophotometric and turbimetric methods for measuring proteins. Methods Enzymol. 3, 447–454
Leegood, R.C., ap Rees, T (1978) Phosphoenolpyruvate carboxykinase and gluconeogenesis in cotyledons of Curcurbita pepo. Biochim.Biophys. Acta 524, 207–218
Loomis, W.D. (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Methods Enzymol. 31, 528–544
Long, S.P., Incoll, L.D., Woolhouse, H.W. (1975) C4 photosynthesis in plants from cool temperate regions, with particular reference to Spartina townsendii. Nature (London) 257, 622–624
Long, S.P., Woolhouse, H.W. (1978a) The responses of net photosynthesis to vapour pressure deficit and CO2 concentration in Spartina townsendii (sensu lato), a C4 species from a cool temperate climate. J. Exp. Bot. 29, 567–577
Long, S.P., Woolhouse, H.W. (1978b) The responses of net photosynthesis to light and temperature in Spartina townsendii (sensuo lato), a C4 species from a cool temperate climate. J. Exp. Bot. 29, 803–814
Lorimer, G.H., Badger, M.R., Andrews, T.J. (1977) D-Ribulose-1,5-bisphosphate carboxylase-oxygenase. Improved methods for the activation and assay of catalytic activities. Anal. Biochem. 78, 66–75
Lowry, O.H., Passonneau, J.V. (1972) A flexible system of enzymatic analysis. Academic Press, New York
Rathnam, C.K.M., Edwards, G.E. (1977) C4-dicarboxylic acid decarboxylation in bundle-sheath chloroplasts, mitochondria and strands of Eriochloa borumensis Hack., a phosphoenolpyruvate-carboxykinase type C4 species. Planta 133, 135–144 (1977)
Sellschop, J.P.F., Salmon, S.C. (1928) The influence of chilling. above the freezing point, on certain crop plants. J. Agric. Res. 37, 315–338
Slabas, A.R., Walker, D.A. (1976) Inhibition of spinach phosphoribulokinase by DL-glyceraldehyde. Biochem. J. 153, 613–619
Taylor, A.O., Rowley, J.A. (1971) Plants under climatic stress. I. Low temperature, high light effects on photosynthesis. Plant Physiol. 47, 713–718
Thomas, S.M., Long, S.P. (1978) C4 photosynthesis in Spartina townsendii at low and high temperatures. Planta 142, 171–174
Wong, K.F., Davies, D.D. (1973) Regulation of phosphoenolpyruvate carboxylase of Zea mays by metabolites. Biochem. J. 131, 451–458
Wu, R., Racker, E. (1959) Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumour cells. J. Biol. Chem. 234, 1029–1035
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Smith, A.M., Woolhouse, H.W. & Jones, D.A. Photosynthetic carbon metabolism of the cool-temperate C4 grass Spartina anglica Hubb.. Planta 156, 441–448 (1982). https://doi.org/10.1007/BF00393315
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DOI: https://doi.org/10.1007/BF00393315