Abstract
Rapid suppression of hypocotyl elongation by blue light in cucumber (Cucumis sativus L.) was studied to examine possible hydraulic and wall changes responsible for diminished growth. Cell-sap osmotic pressure, measured by vaporpressure osmometry, was not decreased by blue light; turgor pressure, measured by the pressureprobe technique, remained constant during the growth inhibition; and stem hydraulic conductance, measured by dynamic and static methods, was likewise unaffected by blue light. Wall yielding properties were assessed by the pressure-block technique for in-vivo stress relaxation. Blue light reduced the initial rate of relaxation by 77%, but had little effect on the final amount of relaxation. The results demonstrate that blue irradiation acts to decrease the wall yielding coefficient, but not the yield threshold. Stress-strain (Instron) analysis showed that irradiation of the seedlings had little effect on the mechanical extensibilities of the isolated wall. The results indicate that blue light can reduce cell-wall loosening without affecting bulk viscoelastic properties, and indicate a chemorheological mechanism of cell-wall expansion.
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Abbreviations
- BL:
-
blue light
- Φ:
-
wall yield coefficient
- Y:
-
wall yield threshold
- P:
-
turgor pressure
- L:
-
hydraulic conductance
- ΔΨg:
-
radial water-potential gradient supporting cell expansion
- π:
-
osmotic pressure
- Pi :
-
initial chamber pressure needed to stop growth
- Pf :
-
final chamber pressure needed to stop growth
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Cosgrove, D.J. Mechanism of rapid suppression of cell expansion in cucumber hypocotyls after blue-light irradiation. Planta 176, 109–116 (1988). https://doi.org/10.1007/BF00392486
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DOI: https://doi.org/10.1007/BF00392486