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Abscisic acid metabolism —vacuolar/extravacuolar distribution of metabolites

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Abstract

The biotransformation of abscisic acid (ABA) was studied in cell suspension cultures of Lycopersicon esculentum. The ABA was converted by the cells to phaseic acid, nigellic acid, dihydrophaseic acid, abscisic acid-β-D-glucopyranosyl ester (ABA-Glc) and other ABA and phaseic acid conjugates. Investigation of their cellular distribution showed that the conjugated forms were located only in the vacuoles whereas ABA and its acidic metabolites were found mainly in the extravacuolar fractions. Our results, together with a number of studies on the increase of ABA-Glc as a response to stress, allow us to propose that ABA-Glc is irreversibly compartmented in the vacuoles of plant cells.

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Abbreviations

ABA:

abscisic acid

ABA-Glc:

β-D-glucopyranosyl ester of ABA

DPA:

4′-dihydrophaseic acid; nigellic acid=3-methyl-5-(1′-hydroxy-2′-hydroxymethyl-6′-dimethyl-4′-oxo-cyclohex-2′-enyl)-penta-2Z, 4E-dienoic acid

PA:

phaseic acid

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Authors and Affiliations

  1. Institute of Plant Biochemistry, Academy of Sciences of the GDR, Weinberg 3, DDR-4010, Halle, German Democratic Republic

    H. Lehmann

  2. Sektion Biowissenschaften, WB Biochemie, Pflanzenbiochemische Abteilung, Martin-Luther-Universität Halle-Wittenberg, Neuwerk 1, DDR-4020, Halle, German Democratic Republic

    K. Glund

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  1. H. Lehmann
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  2. K. Glund
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Lehmann, H., Glund, K. Abscisic acid metabolism —vacuolar/extravacuolar distribution of metabolites. Planta 168, 559–562 (1986). https://doi.org/10.1007/BF00392276

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  • DOI: https://doi.org/10.1007/BF00392276

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