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Exogenous polyamines alter membrane fluidity in bean leaves — a basis for potential misinterpretation of their true physiological role

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Abstract

Changes in the rotational motion of paramagnetic and fluorescent lipid-soluble probes were used to assess the effects of putrescine, spermidine and spermine on the fluidity of microsomal membranes from primary leaves of bean (Phaseolus vulgaris L.). Surface probes were more strongly immobilized by physiological concentrations of the polyamines than probes that partitioned deep into the bilayer interior. Spermidine and spermine were more effective than putrescine at reducing membrane fluidity, and at equimolar concentrations, the polyamines and calcium had similar effects on the mobility of the membrane probes. Spermine had essentially equivalent effects on the fluidity of native membranes, heat-denatured membranes and liposomes prepared from the total lipid extract of the membranes, indicating that polyamines associate with membrane lipid. These results raise the possibility that some of the physiological effects previously attributed to exogenously added polyamines could reflect membrane rigidification rather than a true physiological response.

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Abbreviations

DPH:

diphenylhexatriene

Hepes:

4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid

I-1,14:

16-doxylstearic acid

TMA-DPH:

trimethylammonium diphenylhexatriene

18-NP:

3-(octadecylaminomethyl)-2,2,5,5-tetramethyl-1-pyrrolidinyl-oxyl

τc :

rotational correlation time

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, N2L 3Gl, Waterloo, Ont., Canada

    D. R. Roberts, E. B. Dumbroff & J. E. Thompson

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  1. D. R. Roberts
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  2. E. B. Dumbroff
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  3. J. E. Thompson
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Roberts, D.R., Dumbroff, E.B. & Thompson, J.E. Exogenous polyamines alter membrane fluidity in bean leaves — a basis for potential misinterpretation of their true physiological role. Planta 167, 395–401 (1986). https://doi.org/10.1007/BF00391345

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  • DOI: https://doi.org/10.1007/BF00391345

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