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Gas chromatography-mass spectrometric determinations of abscisic acid levels in the cap and the apex of maize roots

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Abstract

Quantitative analyses of abscisic acid (ABA) in different parts of maize root tips (Zea mays L. cv. Kelvedon 33) were performed by mass fragmentography using the hexadeuterated analog of ABA as internal standard. It was found that the cap and the apex contained 36.1 μg and 66.5 μg ABA kg−1 fresh weight, respectively. The possibility that the growth regulator formed in the cap and inhibiting the elongation of the extending zone of the root is ABA is discussed.

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Abbreviations

ABA:

abscisic acid

ABA-D6 :

hexadeuterated ABA

ABA-Me and ABA-D6-Me:

methyl esters of ABA and ABA-D6, respectively

GC-MS:

gas chromatograph(y)-mass spectrometry/spectrometer

IAA:

indol-3-yl-acetic acid

MF:

mass fragmentography

TMS:

trimethylsilyl

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Authors and Affiliations

  1. Institute of Plant Biology and Physiology of the University, Place de la Riponne, CH-1005, Lausanne, Switzerland

    L. Rivier & P.-E. Pilet

  2. Research Department, Biological Laboratories, Nestlé Products Technical Assistance Company Ltd., CH-1350, Orbe, Switzerland

    H. Milon

Authors
  1. L. Rivier
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  2. H. Milon
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  3. P.-E. Pilet
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Rivier, L., Milon, H. & Pilet, PE. Gas chromatography-mass spectrometric determinations of abscisic acid levels in the cap and the apex of maize roots. Planta 134, 23–27 (1977). https://doi.org/10.1007/BF00390089

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  • DOI: https://doi.org/10.1007/BF00390089

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