Abstract
The binding of fusicoccin to the microsomal preparations of maize roots in vitro is increased several-fold when segments of the tissue are washed for 2 h in distilled water before homogenization. Addition of freeze-dried wash solution to microsomal preparations of spinach leaves or fresh roots, washed roots, or coleoptiles of maize inhibited the binding of fusicoccin to particulate fractions. The freeze-dried material also blocked fusicoccin-promoted H+ extrusion from maize root segments. Roots may contain one or more water-soluble compounds competing with fusicoccin at the receptor level; such ligands might play a physiological role as modulators of the H+/K+ exchange system in higher plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- FC:
-
Fusicoccin
References
Ballio, A.: Fusicoccin: structure-activity relationship. In: Regulation of Cell membrane Activities in Plants, pp. 217–223. Marrè E., Ciferri, O., eds. Amsterdam: Elsevier 1977
Ballio, A.: Chemistry and plant growth regulating activity of fusicoccin derivatives and analogues. In: Advances in Pesticide Science, pt. 2, pp. 336–372. Geissbühler, H., ed. Oxford, New York: Pergamon Press 1979
Ballio, A., D'Alessio, V., Randazzo, G., Bottalico, A., Graniti, A., Sparapano, L., Bosnar, B., Casinovi, G.C., Gribanovski-Sassu, O.: Occurrence of fusicoccin in plant tissues infected by Fusicoccum amygdali Del. Physiol. Plant Pathol. 8, 163–169 (1976)
Ballio, A., Carilli, A., Santurbano, B., Tuttobello, L.: Produzione di fusicoccina in scala pilota. Ann. Ist. Super. Sanità 4, 317–332 (1968)
Ballio, A., Federico, R., Pessi, A., Scalorbi, D.: Fusicoccin binding sites in subcellular preparations of spinach leaves. Plant Sci. Lett. (in press)
Ballio, A., Federico, R., Scalorbi, D.: Metabolic stability of fusicoccin in plant tissues. Rend. Accad. Naz. Lincei 63, 604–608 (1977)
Dohrman, U., Hertel, R., Pesci, P., Cocucci, S., Marrè, E., Randazzo, G., Ballio, A.: Localization of in vitro binding of the fungal toxin fusicoccin to plasma membrane-rich fractions from corn coleoptiles. Plant Sci. Lett. 9, 291–299 (1977)
Gronewald, J.W., Cheeseman, J.M., Hanson, J.B.: Comparison of the responses of corn root tissue to fusicoccin and washing. Plant Physiol. 63, 255–259 (1979)
Lees, M.B., Paxman, S.: Modification of the Lowry procedure for the analysis of proteolipid protein. Anal. Biochem. 47, 184–192 (1972)
Leonard, R.T., Hanson, J.B.: Induction and development of increased ion absorption in corn root tissue. Plant Physiol. 49, 430–435 (1972a)
Leonard, R.T., Hanson, J.B.: Increased membrane-bound adenosine triphosphatase activity accompanying development of enhanced solute uptake in washed corn root tissue. Plant Physiol. 49, 436–440 (1972b)
Lin, W., Hanson, J.B.: Increase in electrogenic membrane potential with washing of corn root tissue. Plant Physiol. 54, 799–801 (1974)
Marrè, E.: Fusicoccin, a tool in plant physiology. Annu. Rev. Plant Physiol. 30, 273–288 (1979)
Marrè, E., Lado, P., Rasi-Caldogno, F., Colombo, R., De Michelis, M.I.: Evidence for the coupling of proton extrusion to K+ uptake in pea internode segments treated with fusicoccin or auxin. Plant Sci. Lett. 3, 365–379 (1974)
Pesci, P., Cocucci, S., Randazzo, G.: Characterization of fusicoccin binding to receptor sites on cell membranes of maize coleoptile tissues. Plant Cell Environm. 3, 205–209 (1979)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aducci, P., Crosetti, G., Federico, R. et al. Fusicoccin receptors. Evidence for endogenous ligand. Planta 148, 208–210 (1980). https://doi.org/10.1007/BF00380028
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00380028