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Glycerolysis of olive oil: batch operational stability of Candida rugosa lipase immobilized in hydrophilic polyurethane foams

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Abstract

The operational stability of the Candida rugosa lipase immobilized in a hydrophilic polyurethane foam was evaluated in consecutive batches for the glycerolysis of olive oil in n-hexane, aimed at the production of monoglycerides.

Glycerol controlled the glycerolysis in the system under study, since it is both a substrate and a powerful water binder that reduces the water activity of the reaction medium and of the microenvironment. Two sets of experiments were carried out under different glycerol/triglyceride ratios. After 345 hours of consecutive 23 hours batches no lipase inactivation was observed.

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Abbreviations

aw :

thermodynamic activity of water

DG:

diglyceride (s)

FAME:

fatty acid methyl ester (s)

FFA:

free fatty acid (s)

FID:

flame ionization detector Gly glycerol

MG:

monoglyceride (s)

TG:

triglyceride (s)

TLC:

thin layer chromatography

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Authors and Affiliations

  1. Laboratório Ferreira Lapa, Instituto Superior de Agronomia, Tapada da Ajuda, 1399, Lisboa, Codex, Portugal

    S. Ferreira-Dias

  2. Instituto Superior Técnico, Laboratório de Eng. Bioquímica, Av Rovisco Pais, 1096, Lisboa, Codex, Portugal

    M. M. R. da Fonseca

Authors
  1. S. Ferreira-Dias
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  2. M. M. R. da Fonseca
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Additional information

The authors are grateful to Profs. P. Adlercreutz, Technical University of Lund, Sweden, and J.M.S. Cabral, Instituto Superior Técnico, Lisbon, Portugal, for inspiring discussions and advice, to Prof. Helena Pereira, Instituto Superior de Agronomia (ISA), Lisbon, Portugal, for the use of GC equipment and to Mrs. Marlene Dionísio, ISA, for invaluable help with some of the experimental work.

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Ferreira-Dias, S., da Fonseca, M.M.R. Glycerolysis of olive oil: batch operational stability of Candida rugosa lipase immobilized in hydrophilic polyurethane foams. Bioprocess Engineering 13, 311–315 (1995). https://doi.org/10.1007/BF00369563

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