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DNA synthesized at pachytene in Lilium: A non-divergent subclass of moderately repetitive sequences

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Abstract

Moderately repetitive sequences of Lilium DNA synthesized during pachytene consist of families that have a considerably lower divergence than those of total genomic middle repeat DNA, the latter having an average divergence of 10%. 80% of the sequences synthesized during the early phase of pachytene and 100% of those synthesized during the latter phase of pachytene reassociate with perfect or near-perfect fidelity. Except for the small amount of DNA synthesized during early pachytene, pachytene middle repeat sequences are non-divergent and thus constitute a distinctive subset of total moderately repetitive DNA. The modal length of pachytene and total middle repeat sequences are similar. In contrast to earlier measurements based on isotope incorporation, the modal length is of the order of 1500–2000 base pairs, ten times the previously estimated size. Calculations based on the new length lead to the conclusion that pachytene middle repeat sequences account for 1% of the genome.

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Bouchard, R.A., Stern, H. DNA synthesized at pachytene in Lilium: A non-divergent subclass of moderately repetitive sequences. Chromosoma 81, 349–363 (1980). https://doi.org/10.1007/BF00368147

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